Evidence map›Paper›PMID 26251247›Full record

Trial reportStroke2015

Remote Ischemic Conditioning Alters Methylation and Expression of Cell Cycle Genes in Aneurysmal Subarachnoid Hemorrhage.

Elina Nikkola, Azim Laiwalla, Arthur Ko, Marcus Alvarez, Mark Connolly, Yinn Cher Ooi, William Hsu, Alex Bui, Päivi Pajukanta, Nestor R Gonzalez

Registry-linked trialOpen access · greenAbstract readClinical Trial
PubMed Full text at PMC Publisher
In one paragraph

Trial report in Stroke, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT02910180 (Genetic, Metabolic, and Growth Factor Repository for Cerebrovascular Disorders), which is not on this map. Cited by 22 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed, 1 pooled it
1.3field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT02910180 recruitingnot on this mapstarted 2016, after this paper: background citation

Genetic, Metabolic, and Growth Factor Repository for Cerebrovascular Disorders

Typeobservational_patient_registrySponsorCedars-Sinai Medical CenterRan2016 to 2030Enrolled900ConditionsCerebrovascular Diseases, Ischemic Stroke, Repository
3 · Its place in the literature

Who cites it

22 citing papers in PubMed, 1 synthesis or guideline pooled it, 31 citations in OpenAlex.

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  9. Conditioning-based therapeutics for aneurysmal subarachnoid hemorrhage - A critical review.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2024
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 2 countries.

Elina NikkolaFrom the Department of Human Genetics (E.N., A.K., M.A., P.P.), Department of Neurosurgery (A.L., M.C., Y.C.O., N.R.G.), and Department of Radiological Sciences (W.H., A.B., N.R.G.), David Geffen School of Medicine at UCLA, Los Angeles, CA; and Department of Human Genetics and Molecular Biology, Molecular Biology Institute at UCLA, Los Angeles, CA (A.K., P.P.).
Azim LaiwallaFrom the Department of Human Genetics (E.N., A.K., M.A., P.P.), Department of Neurosurgery (A.L., M.C., Y.C.O., N.R.G.), and Department of Radiological Sciences (W.H., A.B., N.R.G.), David Geffen School of Medicine at UCLA, Los Angeles, CA; and Department of Human Genetics and Molecular Biology, Molecular Biology Institute at UCLA, Los Angeles, CA (A.K., P.P.).
Arthur KoFrom the Department of Human Genetics (E.N., A.K., M.A., P.P.), Department of Neurosurgery (A.L., M.C., Y.C.O., N.R.G.), and Department of Radiological Sciences (W.H., A.B., N.R.G.), David Geffen School of Medicine at UCLA, Los Angeles, CA; and Department of Human Genetics and Molecular Biology, Molecular Biology Institute at UCLA, Los Angeles, CA (A.K., P.P.).
Marcus AlvarezFrom the Department of Human Genetics (E.N., A.K., M.A., P.P.), Department of Neurosurgery (A.L., M.C., Y.C.O., N.R.G.), and Department of Radiological Sciences (W.H., A.B., N.R.G.), David Geffen School of Medicine at UCLA, Los Angeles, CA; and Department of Human Genetics and Molecular Biology, Molecular Biology Institute at UCLA, Los Angeles, CA (A.K., P.P.).
Mark ConnollyFrom the Department of Human Genetics (E.N., A.K., M.A., P.P.), Department of Neurosurgery (A.L., M.C., Y.C.O., N.R.G.), and Department of Radiological Sciences (W.H., A.B., N.R.G.), David Geffen School of Medicine at UCLA, Los Angeles, CA; and Department of Human Genetics and Molecular Biology, Molecular Biology Institute at UCLA, Los Angeles, CA (A.K., P.P.).
Yinn Cher OoiFrom the Department of Human Genetics (E.N., A.K., M.A., P.P.), Department of Neurosurgery (A.L., M.C., Y.C.O., N.R.G.), and Department of Radiological Sciences (W.H., A.B., N.R.G.), David Geffen School of Medicine at UCLA, Los Angeles, CA; and Department of Human Genetics and Molecular Biology, Molecular Biology Institute at UCLA, Los Angeles, CA (A.K., P.P.).
William HsuFrom the Department of Human Genetics (E.N., A.K., M.A., P.P.), Department of Neurosurgery (A.L., M.C., Y.C.O., N.R.G.), and Department of Radiological Sciences (W.H., A.B., N.R.G.), David Geffen School of Medicine at UCLA, Los Angeles, CA; and Department of Human Genetics and Molecular Biology, Molecular Biology Institute at UCLA, Los Angeles, CA (A.K., P.P.).
Alex BuiFrom the Department of Human Genetics (E.N., A.K., M.A., P.P.), Department of Neurosurgery (A.L., M.C., Y.C.O., N.R.G.), and Department of Radiological Sciences (W.H., A.B., N.R.G.), David Geffen School of Medicine at UCLA, Los Angeles, CA; and Department of Human Genetics and Molecular Biology, Molecular Biology Institute at UCLA, Los Angeles, CA (A.K., P.P.).
Päivi PajukantaFrom the Department of Human Genetics (E.N., A.K., M.A., P.P.), Department of Neurosurgery (A.L., M.C., Y.C.O., N.R.G.), and Department of Radiological Sciences (W.H., A.B., N.R.G.), David Geffen School of Medicine at UCLA, Los Angeles, CA; and Department of Human Genetics and Molecular Biology, Molecular Biology Institute at UCLA, Los Angeles, CA (A.K., P.P.).
Nestor R GonzalezFrom the Department of Human Genetics (E.N., A.K., M.A., P.P.), Department of Neurosurgery (A.L., M.C., Y.C.O., N.R.G.), and Department of Radiological Sciences (W.H., A.B., N.R.G.), David Geffen School of Medicine at UCLA, Los Angeles, CA; and Department of Human Genetics and Molecular Biology, Molecular Biology Institute at UCLA, Los Angeles, CA (A.K., P.P.). ngonzalez@mednet.ucla.edu.
University of California, Los Angeles · US

Funding

Systems genomics of metabolic syndrome traitsP01HL028481 · NHLBI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI LUSIS, ALDONS JAKE · 1985 to 2019
$50.2M
UCLA MSD ProgramR25GM055052 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI BARBER, PAUL HENRY, HASSON, TAMA W. · 1996 to 2021
$9.2M
Training Grant in Genomic Analysis and InterpretationT32HG002536 · NHGRI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Valerie A Arboleda, Harold Pimentel · 2002 to 2026
$8.6M
Genomics of dyslipidemia in MexicansR01HL095056 · NHLBI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI PAJUKANTA, PAIVI · 2009 to 2018
$5.9M
Toward Individually-tailored Medicine: Probabilistic Models of Cerebral AneurysmsR01EB000362 · NIBIB · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI BUI, ALEX · 2003 to 2012
$5.1M
Surgical Indirect Revascularization For Symptomatic Intracranial Aterial StenosisK23NS079477 · NINDS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI GONZALEZ, NESTOR RAUL · 2013 to 2017
$842k
NHGRI NIH HHS T32 HG002536NHLBI NIH HHS HL-095056NHLBI NIH HHS HL-28481NHLBI NIH HHS P01 HL028481NHLBI NIH HHS R01 HL095056NIBIB NIH HHS R01 EB000362NIBIB NIH HHS R01EB000362NIGMS NIH HHS R25 GM055052NINDS NIH HHS K23 NS079477NINDS NIH HHS K23NS079477
6 · The paper itself

Abstract

background and purposeRemote ischemic conditioning (RIC) is a phenomenon in which short periods of nonfatal ischemia in 1 tissue confers protection to distant tissues. Here we performed a longitudinal human pilot study in patients with aneurysmal subarachnoid hemorrhage undergoing RIC by limb ischemia to compare changes in DNA methylation and transcriptome profiles before and after RIC.

methodsThirteen patients underwent 4 RIC sessions over 2 to 12 days after rupture of an intracranial aneurysm. We analyzed whole blood transcriptomes using RNA sequencing and genome-wide DNA methylomes using reduced representation bisulfite sequencing, both before and after RIC. We tested differential expression and differential methylation using an intraindividual paired study design and then overlapped the differential expression and differential methylation results for analyses of functional categories and protein-protein interactions.

resultsWe observed 164 differential expression genes and 3493 differential methylation CpG sites after RIC, of which 204 CpG sites overlapped with 103 genes, enriched for pathways of cell cycle (P<3.8×10(-4)) and inflammatory responses (P<1.4×10(-4)). The cell cycle pathway genes form a significant protein-protein interaction network of tightly coexpressed genes (P<0.00001).

conclusionsGene expression and DNA methylation changes in aneurysmal subarachnoid hemorrhage patients undergoing RIC are involved in coordinated cell cycle and inflammatory responses.

Indexed as

AdultAgedDNA MethylationFemaleGene ExpressionGenes, cdcHumansIntracranial AneurysmIschemic PreconditioningLongitudinal StudiesMaleMiddle AgedPilot ProjectsSubarachnoid HemorrhageTranscriptomeaneurysmDNA methylationgenomicspreconditioningsubarachnoid hemorrhagetranscriptome

Identifiers

PMID26251247
PMCPMC4550559
OpenAlexW1915140892

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Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.