Evidence map›Paper›PMID 26250446›Full record

ArticleVirus genes2015

The RNA-binding properties and domain of Rice stripe virus nucleocapsid protein.

Shuling Zhao, Yanan Xue, Jiahui Hao, Changyong Liang

Abstract read
PubMed Publisher
In one paragraph

Article in Virus genes, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
2.1field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 5 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Shuling ZhaoCollege of Bioscience and Biotechnology, Yangzhou University, No. 12 Wenhui Road East, Yangzhou, 225009, People's Republic of China. slzhao@yzu.edu.cn.
Yanan XueCollege of Bioscience and Biotechnology, Yangzhou University, No. 12 Wenhui Road East, Yangzhou, 225009, People's Republic of China.
Jiahui HaoCollege of Bioscience and Biotechnology, Yangzhou University, No. 12 Wenhui Road East, Yangzhou, 225009, People's Republic of China.
Changyong LiangCollege of Bioscience and Biotechnology, Yangzhou University, No. 12 Wenhui Road East, Yangzhou, 225009, People's Republic of China.
Yangzhou University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The nucleocapsid protein (NP) of rice stripe virus (RSV) encapsidates viral genomic RNAs to form virion. The binding of NP with RNA is essential for the formation of virus particle. In this study, the binding specificity of RSV NP to RNA and the domains within the NP that mediate this interaction were investigated by gel electrophoretic mobility shift assays and Northwestern blot analysis. The results demonstrated that RSV NP was able to bind to all synthetic RNAs and DNAs without sequence specificity. Using a series of truncated NPs expressed in E. coli and synthetic peptides, we mapped the RNA-binding domain of NP to the central region from amino acid residues 201-232. Further alanine substitution analysis revealed that Lys(206), Lys(207), Lys(220), and Tyr(221) in the RNA-binding domain were essential for NP to bind with RNA.

Indexed as

Blotting, NorthernDNADNA Mutational AnalysisElectrophoretic Mobility Shift AssayEscherichia coliGene ExpressionMutagenesis, Site-DirectedNucleocapsid ProteinsProtein BindingProtein Structure, TertiaryRNARNA-Binding ProteinsSequence DeletionSubstrate SpecificityTenuivirusDNANucleocapsid ProteinsRNARNA-Binding ProteinsBinding domainEMSANucleocapsid proteinRice stripe virus

Identifiers

PMID26250446
OpenAlexW1119279508

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.