ArticleActa crystallographica. Section D, Biological crystallography2015
Defined PEG smears as an alternative approach to enhance the search for crystallization conditions and crystal-quality improvement in reduced screens.
Article in Acta crystallographica. Section D, Biological crystallography, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
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- Benzoic and salicylic acids inhibit β-substituted alanine synthase 4;1 in common bean.Plant physiology · 2025Article
- ARR1 and AHP interactions in the multi-step phosphorelay system.Frontiers in plant science · 2025Article
- Identifying novel chemical matter against the Chikungunya virus nsP3 macrodomain through crystallographic fragment screening.bioRxiv : the preprint server for biology · 2024Article
- The H163A mutation unravels an oxidized conformation of the SARS-CoV-2 main protease.Nature communications · 2023Article
- A recurring packing contact in crystals of InlB pinpoints functional binding sites in the internalin domain and the B repeat.Acta crystallographica. Section D, Structural biology · 2022Article
- Structure of the unique tetrameric STENOFOLIA homeodomain bound with target promoter DNA.Acta crystallographica. Section D, Structural biology · 2021Article
- Structural Insights into Plasticity and Discovery of Remdesivir Metabolite GS-441524 Binding in SARS-CoV-2 Macrodomain.ACS medicinal chemistry letters · 2021Article
- The copper(II)-binding tripeptide GHK, a valuable crystallization and phasing tag for macromolecular crystallography.Acta crystallographica. Section D, Structural biology · 2020Article
- Comprehensive Set of Tertiary Complex Structures and Palmitic Acid Binding Provide Molecular Insights into Ligand Design for RXR Isoforms.International journal of molecular sciences · 2020Article
- Receptor-interacting protein kinase 2 (RIPK2) and nucleotide-binding oligomerization domain (NOD) cell signaling inhibitors based on a 3,5-diphenyl-2-aminopyridine scaffold.European journal of medicinal chemistry · 2020Article
- Structural Characterization of anBiomolecules · 2020Article
- A Searchable Database of Crystallization Cocktails in the PDB: Analyzing the Chemical Condition Space.Patterns (New York, N.Y.) · 2020Article
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- Crystal Structure of the LSD1/CoREST Histone Demethylase Bound to Its Nucleosome Substrate.Molecular cell · 2020Article
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- Crystal structure of UDP-glucose pyrophosphorylase from Yersinia pestis, a potential therapeutic target against plague.Acta crystallographica. Section F, Structural biology communications · 2019Article
- Functional assembly of nitrous oxide reductase provides insights into copper site maturation.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
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3 authors.
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Abstract
The quest for an optimal limited set of effective crystallization conditions remains a challenge in macromolecular crystallography, an issue that is complicated by the large number of chemicals which have been deemed to be suitable for promoting crystal growth. The lack of rational approaches towards the selection of successful chemical space and representative combinations has led to significant overlapping conditions, which are currently present in a multitude of commercially available crystallization screens. Here, an alternative approach to the sampling of widely used PEG precipitants is suggested through the use of PEG smears, which are mixtures of different PEGs with a requirement of either neutral or cooperatively positive effects of each component on crystal growth. Four newly defined smears were classified by molecular-weight groups and enabled the preservation of specific properties related to different polymer sizes. These smears not only allowed a wide coverage of properties of these polymers, but also reduced PEG variables, enabling greater sampling of other parameters such as buffers and additives. The efficiency of the smear-based screens was evaluated on more than 220 diverse recombinant human proteins, which overall revealed a good initial crystallization success rate of nearly 50%. In addition, in several cases successful crystallizations were only obtained using PEG smears, while various commercial screens failed to yield crystals. The defined smears therefore offer an alternative approach towards PEG sampling, which will benefit the design of crystallization screens sampling a wide chemical space of this key precipitant.
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