ArticleArthritis research & therapy2015
Peripheral blood derived mononuclear cells enhance osteoarthritic human chondrocyte migration.
Article in Arthritis research & therapy, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 1 of them a synthesis that pooled it.
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Who cites it
16 citing papers in PubMed, 1 synthesis or guideline pooled it, 30 citations in OpenAlex.
- Peripheral Blood-Derived Stem Cells for the Treatment of Cartilage Injuries: A Systematic Review.Frontiers in bioengineering and biotechnology · 2022Pooled it
- Tetrahedral framework nucleic acids: Nanokeys unlocking a new era of precision biomedicine.Journal of advanced research · 2026Review
- A six-gene expression signature in peripheral blood mononuclear cells effectively diagnoses osteoarthritis.Frontiers in medicine · 2025Article
- Functional and Molecular Analysis of Human Osteoarthritic Chondrocytes Treated with Bone Marrow-Derived MSC-EVs.Bioengineering (Basel, Switzerland) · 2024Article
- Chondrocyte Thrombomodulin Protects against Osteoarthritis.International journal of molecular sciences · 2023Article
- NG2/CSPG4 attenuates motility in mandibular fibrochondrocytes under serum starvation conditions.Frontiers in cell and developmental biology · 2023Article
- The Function of Metformin in Aging-Related Musculoskeletal Disorders.Frontiers in pharmacology · 2022Review
- Research trends of mesenchymal stem cells application in orthopedics: A bibliometric analysis of the past 2 decades.Frontiers in public health · 2022Article
- Using apheresis-derived cells to augment microdrilling in the treatment of chondral defects in an ovine model.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2021Article
- Narrative review of the choices of stem cell sources and hydrogels for cartilage tissue engineering.Annals of translational medicine · 2020Review
- Extracellular Nucleotides Selectively Induce Migration of Chondrocytes and Expression of Type II Collagen.International journal of molecular sciences · 2020Article
- The Use of Peripheral Blood-Derived Stem Cells for Cartilage Repair and RegenerationFrontiers in pharmacology · 2020Review
- Real-time cell analysis system in cytotoxicity applications: Usefulness and comparison with tetrazolium salt assays.Toxicology reports · 2020Review
- Article
- Peripheral mononuclear blood cell apheresis in a preclinical ovine model.BMC veterinary research · 2018Article
- Regenerative medicine. PBMCs stimulate chondrocyte migration and cartilage repair.Nature reviews. Rheumatology · 2015Article
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6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionA major problem in cartilage repair is the lack of chondrogenic cells migrating from healthy tissue into defects. Cartilage is essentially avascular and therefore its healing is not considered to involve mononuclear cells. Peripheral blood derived mononuclear cells (PBMC) offer a readily available autologous cell source for clinical use and therefore this study was designed to evaluate the effects of PBMCs on chondrocytes and cartilage.
methodsHuman primary chondrocytes and cartilage tissue explants were taken from patients undergoing total knee replacement (n = 17). Peripheral blood samples were obtained from healthy volunteers (n = 12) and mononuclear cells were isolated by density-gradient centrifugation. Cell migration and chemokinetic potential were measured using a scratch assay, xCELLigence and CyQuant assay. PCR array and quantitative PCR was used to evaluate mRNA expression of 87 cell motility and/or chondrogenic genes.
resultsThe chondrocyte migration rate was 2.6 times higher at 3 hour time point (p < 0.0001) and total number of migrating chondrocytes was 9.7 times higher (p < 0.0001) after three day indirect PBMC stimulus and 8.2 times higher (p < 0.0001) after three day direct co-culture with PBMCs. A cartilage explant model confirmed that PBMCs also exert a chemokinetic role on ex vivo tissue. PBMC stimulation was found to significantly upregulate the mRNA levels of 2 chondrogenic genes; collagen type II (COL2A1 600-fold, p < 0.0001) and SRY box 9 (SOX9 30-fold, p < 0.0001) and the mRNA levels of 7 genes central in cell motility and migration were differentially regulated by 24h PBMC stimulation.
conclusionThe results support the concept that PBMC treatment enhances chondrocyte migration without suppressing the chondrogenic phenotype possibly via mechanistic pathways involving MMP9 and IGF1. In the future, peripheral blood mononuclear cells could be used as an autologous point-ofcare treatment to attract native chondrocytes from the diseased tissue to aid in cartilage repair.
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