ArticleAntimicrobial agents and chemotherapy2015
Detection of a New cfr-Like Gene, cfr(B), in Enterococcus faecium Isolates Recovered from Human Specimens in the United States as Part of the SENTRY Antimicrobial Surveillance Program.
Article in Antimicrobial agents and chemotherapy, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers.
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68 citing papers in PubMed, 159 citations in OpenAlex.
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- Global dissemination of optrA-mediated linezolid resistance in enterococci.The Journal of antimicrobial chemotherapy · 2026Article
- Environmental occurrence of optrA-mediated linezolid resistance in Enterococcus isolates and genomic insights into Enterococcus faecium ST54 co-harboring optrA, poxtA, and cfr(D) genes.World journal of microbiology & biotechnology · 2026Article
- Global distribution and genomic characteristics of linezolid resistance gene-positiveFrontiers in microbiology · 2026Article
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- Linezolid in the Focus of Antimicrobial Resistance ofInternational journal of molecular sciences · 2025Review
- Molecular Characterization of Linezolid-Non-SusceptibleInfection and drug resistance · 2025Article
- Genetic Characterization of a Linezolid- and Penicillin-ResistantTransboundary and emerging diseases · 2025Article
- Non-Clinical microbiology reviews · 2024Review
- Genomic epidemiology reveals multiple mechanisms of linezolid resistance in clinical enterococci in China.Annals of clinical microbiology and antimicrobials · 2024Article
- Mapping the widespread distribution and transmission dynamics of linezolid resistance in humans, animals, and the environment.Microbiome · 2024Article
- Risk exploration and prediction model construction for linezolid-resistant Enterococcus faecalis based on big data in a province in southern China.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2024Article
- Colocalization of Linezolid Resistance (Antimicrobial agents and chemotherapy · 2023Article
- Linezolid-Resistant Enterococcus faecalis of Chicken Origin Harbored Chromosome-BorneMicrobiology spectrum · 2023Article
- Ribosome-targeting antibiotics and resistanceRSC medicinal chemistry · 2023Review
- Phenotypic and genotypic characterization of linezolid resistance and the effect of antibiotic combinations on methicillin-resistant Staphylococcus aureus clinical isolates.Annals of clinical microbiology and antimicrobials · 2023Article
- Circular intermediate-mediated horizontal transfer of the chromosome-encodedFrontiers in microbiology · 2023Article
- Detection of Genes Related to Linezolid Resistance (Advanced biomedical research · 2023Article
- Genomic characterization of vancomycin-resistantFrontiers in microbiology · 2023Article
8 more citing papers are in PubMed but not listed here.
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7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Two linezolid-resistant Enterococcus faecium isolates (MICs, 8 μg/ml) from unique patients of a medical center in New Orleans were included in this study. Isolates were initially investigated for the presence of mutations in the V domain of 23S rRNA genes and L3, L4, and L22 ribosomal proteins, as well as cfr. Isolates were subjected to pulsed-field gel electrophoresis (just one band difference), and one representative strain was submitted to whole-genome sequencing. Gene location was also determined by hybridization, and cfr genes were cloned and expressed in a Staphylococcus aureus background. The two isolates had one out of six 23S rRNA alleles mutated (G2576T), had wild-type L3, L4, and L22 sequences, and were positive for a cfr-like gene. The sequence of the protein encoded by the cfr-like gene was most similar (99.7%) to that found in Peptoclostridium difficile, which shared only 74.9% amino acid identity with the proteins encoded by genes previously identified in staphylococci and non-faecium enterococci and was, therefore, denominated Cfr(B). When expressed in S. aureus, the protein conferred a resistance profile similar to that of Cfr. Two copies of cfr(B) were chromosomally located and embedded in a Tn6218 similar to the cfr-carrying transposon described in P. difficile. This study reports the first detection of cfr genes in E. faecium clinical isolates in the United States and characterization of a new cfr variant, cfr(B). cfr(B) has been observed in mobile genetic elements in E. faecium and P. difficile, suggesting potential for dissemination. However, further analysis is necessary to access the resistance levels conferred by cfr(B) when expressed in enterococci.
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