Evidence map›Paper›PMID 26247382›Full record

ArticleJournal of colloid and interface science2015

A methacrylate-based polymeric imidazole ligand yields quantum dots with low cytotoxicity and low nonspecific binding.

Colin M Johnson, Kayla M Pate, Yi Shen, Anand Viswanath, Rui Tan, Brian C Benicewicz, Melissa A Moss, Andrew B Greytak

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of colloid and interface science, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Colin M JohnsonDepartment of Chemistry and Biochemistry, University of South Carolina, Columbia, SC 29208, United States.
Kayla M PateDepartment of Chemical Engineering, University of South Carolina, Columbia, SC 29208, United States.
Yi ShenDepartment of Chemistry and Biochemistry, University of South Carolina, Columbia, SC 29208, United States.
Anand ViswanathDepartment of Chemistry and Biochemistry, University of South Carolina, Columbia, SC 29208, United States.
Rui TanDepartment of Chemistry and Biochemistry, University of South Carolina, Columbia, SC 29208, United States.
Brian C BenicewiczDepartment of Chemistry and Biochemistry, University of South Carolina, Columbia, SC 29208, United States; USC Nanocenter, University of South Carolina, Columbia, SC 29208, United States.
Melissa A MossDepartment of Chemical Engineering, University of South Carolina, Columbia, SC 29208, United States; Biomedical Engineering Program, University of South Carolina, Columbia, SC 29208, United States.
Andrew B GreytakDepartment of Chemistry and Biochemistry, University of South Carolina, Columbia, SC 29208, United States; USC Nanocenter, University of South Carolina, Columbia, SC 29208, United States. Electronic address: greytak@sc.edu.

Funding

Targeting early ceramide elevation in pre-symptomatic eczemaP20GM103641 · NIGMS · UNIVERSITY OF SOUTH CAROLINA AT COLUMBIA · PI ENOS, REILLY, NAGARKATTI, PRAKASH S · 2012 to 2023
$20.7M
NIGMS NIH HHS P20 GM103641
6 · The paper itself

Abstract

This paper assesses the biocompatibility for fluorescence imaging of colloidal nanocrystal quantum dots (QDs) coated with a recently-developed multiply-binding methacrylate-based polymeric imidazole ligand. The QD samples were purified prior to ligand exchange via a highly repeatable gel permeation chromatography (GPC) method. A multi-well plate based protocol was used to characterize nonspecific binding and toxicity of the QDs toward human endothelial cells. Nonspecific binding in 1% fetal bovine serum was negligible compared to anionically-stabilized QD controls, and no significant toxicity was detected on 24h exposure. The nonspecific binding results were confirmed by fluorescence microscopy. This study is the first evaluation of biocompatibility in QDs initially purified by GPC and represents a scalable approach to comparison among nanocrystal-based bioimaging scaffolds.

Indexed as

Bio-imagingCdSeCore/shell quantum dotsFluorescence microscopyGel permeation chromatographyLigand exchangeLive cellsNonspecific bindingPolymerToxicity

Identifiers

PMID26247382
PMCPMC4823015

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.