ArticleNature protocols2015
Molecular characterization of neuronal cell types based on patterns of projection with Retro-TRAP.
Article in Nature protocols, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 46 citations in OpenAlex.
- Generalization of negative memories drives the development of psychological distress-related behaviors.Innovation (Cambridge (Mass.)) · 2026Article
- Molecular Profiling of VGluT1 AND VGluT2 Ventral Subiculum to Nucleus Accumbens Shell Projections.Neurochemical research · 2023Article
- Viral tools for neuroscience.Nature reviews. Neuroscience · 2020Review
- Signatures of sex: Sex differences in gene expression in the vertebrate brain.Wiley interdisciplinary reviews. Developmental biology · 2020Review
- Article
- αBiological psychiatry · 2019Article
- Molecular tools to elucidate factors regulating alcohol use.Alcohol (Fayetteville, N.Y.) · 2019Review
- Cell-Type Identification in the Autonomic Nervous System.Neuroscience bulletin · 2019Review
- Molecular profiling of reticular gigantocellularis neurons indicates that eNOS modulates environmentally dependent levels of arousal.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Genetic approaches to access cell types in mammalian nervous systems.Current opinion in neurobiology · 2018Review
- Mapping Molecular Datasets Back to the Brain Regions They are Extracted from: Remembering the Native Countries of Hypothalamic Expatriates and Refugees.Advances in neurobiology · 2018Article
- Combined Social and Spatial Coding in a Descending Projection from the Prefrontal Cortex.Cell · 2017Article
- Reward Circuitry in Addiction.Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics · 2017Review
- Rapid Molecular Profiling of Defined Cell Types Using Viral TRAP.Cell reports · 2017Article
- Gene Expression Profiling with Cre-Conditional Pseudorabies Virus Reveals a Subset of Midbrain Neurons That Participate in Reward Circuitry.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Article
- Diversity matters - heterogeneity of dopaminergic neurons in the ventral mesencephalon and its relation to Parkinson's Disease.Journal of neurochemistry · 2016Review
- Molecular heterogeneity of midbrain dopaminergic neurons--Moving toward single cell resolution.FEBS letters · 2015Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Retro-TRAP (translating ribosome affinity purification) technology enables the synthesis of molecular and neuroanatomical information through the use of transgenic and viral approaches. In contrast to other methods that are used to profile neural circuits such as laser-capture microdissection and FACS, Retro-TRAP is a high-throughput methodology that requires minimal specialized instrumentation. Retro-TRAP uses an anti-GFP ribosomal tag (expressed virally or using transgenesis) to immunoprecipitate translating mRNAs from any population of neurons that express GFP. The protocol detailed here describes the rapid extraction of molecular information from neural circuits in mice using retrograde-tracing GFP-expressing viruses. This approach can be used to identify novel cell types, as well as to molecularly profile cell types for which Cre-driver lines are available, in defined presynaptic loci. The current protocol describes a method for extracting translating mRNA from any neural circuit accessible by stereotaxic injection and manual dissection, and it takes 2-4 weeks. Although it is not described here, this mRNA can then be used in downstream processing applications such as quantitative PCR (qPCR) and high-throughput RNA sequencing to obtain 'molecular connectomic' information.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.