ArticleIranian journal of parasitology
Detection of Acanthamoeba and Toxoplasma in River Water Samples by Molecular Methods in Iran.
Article in Iranian journal of parasitology. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers, 1 of them a synthesis that pooled it.
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Who cites it
11 citing papers in PubMed, 1 synthesis or guideline pooled it, 35 citations in OpenAlex.
- Prevalence of Free-Living Amoebae in Various Water Sources in Iran: A Systematic Review and Meta-Analysis.Acta parasitologica · 2025Pooled it
- Free-Living Amoebae Infections: Case Reports Identified at a Reference Parasitology Laboratory in Argentina.Acta parasitologica · 2025Article
- Review
- Contamination of Soil, Water, Fresh Produce, and Bivalve Mollusks withMicroorganisms · 2022Review
- Investigating the frequency of free-living amoeba in water resources with emphasis on Acanthamoeba in Bandar Abbas city, Hormozgan province, Iran in 2019-2020.BMC research notes · 2020Article
- Detection of human intestinal protozoan parasites in vegetables and fruits: a review.Parasites & vectors · 2020Review
- First molecular detection of Toxoplasma gondii in vegetable samples in China using qualitative, quantitative real-time PCR and multilocus genotyping.Scientific reports · 2019Article
- Real-time PCR detection of Toxoplasma gondii in surface water samples in São Paulo, Brazil.Parasitology research · 2019Article
- Free-living amoebae in the water resources of Iran: a systematic review.Journal of parasitic diseases : official organ of the Indian Society for Parasitology · 2017Review
- More Than Seventy Years of Research (1948-November 2021) onIranian journal of parasitologyReview
- Molecular Identification of Free-Living Amoebae (Iranian journal of parasitologyArticle
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundFree-living amoebae such as Acanthamoeba species may act as carriers of Cryptosporidium and Toxoplasma oocysts, thus, may play an important role in the water-borne transmission of these parasites. In the present study, a loop mediated isothermal amplification (LAMP) method for detection of Toxoplasma and a PCR assay were developed for investigation of Acanthamoeba in environmental water samples.
methodsA total of 34 samples were collected from the surface water in Guilan Province. Water samples were filtrated with membrane filters and followed by DNA extraction. PCR and LAMP methods used for detection of the protozoan parasites Acanthamoeba and Toxoplasma respectively.
resultsTotally 30 and 2 of 34 samples were positive for Acanthamoeba and Toxoplasma oocysts respectively. Two samples were positive for both investigated parasites.
conclusionThe investigated water supplies, are contaminated by Toxoplasma and Acanthamoeba (oo)cystes. Acanthamoeba may play an important role in water-borne transmission of Toxoplasma in the study area. For the first time in Iran, protocol of LAMP method was used effectively for the detection of Toxoplasma in surface water samples in Iran.
Indexed as
Identifiers
26246823PMC4522301W2416375034What OpenQuestion holds
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