ArticleMolecular biology of the cell2015
Autophosphorylation of the Smk1 MAPK is spatially and temporally regulated by Ssp2 during meiotic development in yeast.
Article in Molecular biology of the cell, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 16 citations in OpenAlex.
- Conserved and rewired MAPK networks in human fungal pathogens.FEMS yeast research · 2026Review
- Remodeling of the secretory pathway is coordinated withiScience · 2024Article
- Membrane and organelle rearrangement during ascospore formation in budding yeast.Microbiology and molecular biology reviews : MMBR · 2024Review
- The MAPK homolog, Smk1, promotes assembly of the glucan layer of the spore wall in S. cerevisiae.Yeast (Chichester, England) · 2024Article
- Meiotic Cytokinesis inJournal of fungi (Basel, Switzerland) · 2024Review
- Suppression of Vps13 adaptor protein mutants reveals a central role for PI4P in regulating prospore membrane extension.PLoS genetics · 2021Article
- The Smk1 MAPK and Its Activator, Ssp2, Are Required for Late Prospore Membrane Development in SporulatingJournal of fungi (Basel, Switzerland) · 2021Article
- Isc10, an Inhibitor That Links the Anaphase-Promoting Complex to a Meiosis-Specific Mitogen-Activated Protein Kinase.Molecular and cellular biology · 2020Article
- Novel insights into gene expression regulation during meiosis revealed by translation elongation dynamics.NPJ systems biology and applications · 2019Article
- RNA Recognition-like Motifs Activate a Mitogen-Activated Protein Kinase.Biochemistry · 2018Article
- Yeast can accommodate phosphotyrosine: v-Src toxicity in yeast arises from a single disrupted pathway.FEMS yeast research · 2018Article
- The meiosis-specific Cdc20 family-member Ama1 promotes binding of the Ssp2 activator to the Smk1 MAP kinase.Molecular biology of the cell · 2018Article
- Ssp2 Binding Activates the Smk1 Mitogen-Activated Protein Kinase.Molecular and cellular biology · 2017Article
- Post-transcriptional regulation in budding yeast meiosis.Current genetics · 2016Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Smk1 is a meiosis-specific MAPK that controls spore wall morphogenesis in Saccharomyces cerevisiae. Although Smk1 is activated by phosphorylation of the threonine (T) and tyrosine (Y) in its activation loop, it is not phosphorylated by a dual-specificity MAPK kinase. Instead, the T is phosphorylated by the cyclin-dependent kinase (CDK)-activating kinase, Cak1. The Y is autophosphorylated in an intramolecular reaction that requires a meiosis-specific protein named Ssp2. The meiosis-specific CDK-like kinase, Ime2, was previously shown to positively regulate Smk1. Here we show that Ime2 activity is required to induce the translation of SSP2 mRNA at anaphase II. Ssp2 protein is then localized to the prospore membrane, the structure where spore wall assembly takes place. Next the carboxy-terminal portion of Ssp2 forms a complex with Smk1 and stimulates the autophosphorylation of its activation-loop Y residue. These findings link Ime2 to Smk1 activation through Ssp2 and define a developmentally regulated mechanism for activating MAPK at specific locations in the cell.
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