ArticleOpen biology2015
Dissecting the function of Atg1 complex in Dictyostelium autophagy reveals a connection with the pentose phosphate pathway enzyme transketolase.
Article in Open biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 22 citations in OpenAlex.
- The Multifaceted Interactions ofCells · 2024Article
- Methods to Assess Autophagic Activity in Dictyostelium.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Article
- The proppin Bcas3 and its interactor KinkyA localize to the early phagophore and regulate autophagy.Autophagy · 2021Article
- TheFrontiers in cell and developmental biology · 2021Review
- Calmodulin-mediated events during the life cycle of the amoebozoan Dictyostelium discoideum.Biological reviews of the Cambridge Philosophical Society · 2020Review
- Cyclic AMP induction of Dictyostelium prespore gene expression requires autophagy.Developmental biology · 2019Article
- VPS13A is closely associated with mitochondria and is required for efficient lysosomal degradation.Disease models & mechanisms · 2019Article
- IreA Controls Endoplasmic Reticulum Stress-Induced Autophagy and Survival through Homeostasis Recovery.Molecular and cellular biology · 2018Article
- Methods to Monitor and Quantify Autophagy in the Social Amoeba Dictyostelium discoideum.Cells · 2017Review
- Mycobacterium marinum antagonistically induces an autophagic response while repressing the autophagic flux in a TORC1- and ESX-1-dependent manner.PLoS pathogens · 2017Article
- Autophagy in Dictyostelium: Mechanisms, regulation and disease in a simple biomedical model.Autophagy · 2017Review
- When Dicty Met Myco, a (Not So) Romantic Story about One Amoeba and Its Intracellular Pathogen.Frontiers in cellular and infection microbiology · 2017Review
- Ribose 5-phosphate isomerase inhibits LC3 processing and basal autophagy.Cellular signalling · 2016Article
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Authors and funding
6 authors at 1 institution in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The network of protein-protein interactions of the Dictyostelium discoideum autophagy pathway was investigated by yeast two-hybrid screening of the conserved autophagic proteins Atg1 and Atg8. These analyses confirmed expected interactions described in other organisms and also identified novel interactors that highlight the complexity of autophagy regulation. The Atg1 kinase complex, an essential regulator of autophagy, was investigated in detail here. The composition of the Atg1 complex in D. discoideum is more similar to mammalian cells than to Saccharomyces cerevisiae as, besides Atg13, it contains Atg101, a protein not conserved in this yeast. We found that Atg101 interacts with Atg13 and genetic disruption of these proteins in Dictyostelium leads to an early block in autophagy, although the severity of the developmental phenotype and the degree of autophagic block is higher in Atg13-deficient cells. We have also identified a protein containing zinc-finger B-box and FNIP motifs that interacts with Atg101. Disruption of this protein increases autophagic flux, suggesting that it functions as a negative regulator of Atg101. We also describe the interaction of Atg1 kinase with the pentose phosphate pathway enzyme transketolase (TKT). We found changes in the activity of endogenous TKT activity in strains lacking or overexpressing Atg1, suggesting the presence of an unsuspected regulatory pathway between autophagy and the pentose phosphate pathway in Dictyostelium that seems to be conserved in mammalian cells.
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