ArticleJournal of clinical microbiology2015
Application of Isothermal Amplification Techniques for Identification of Madurella mycetomatis, the Prevalent Agent of Human Mycetoma.
Article in Journal of clinical microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Host genetic susceptibility to mycetoma.PLoS neglected tropical diseases · 2020Pooled it
- Mycetoma.PLoS neglected tropical diseases · 2026Review
- The development of a multiplex recombinase polymerase amplification reaction to detect the most common causative agents of eumycetoma.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2025Article
- Rapid and Accurate Detection ofInsects · 2024Article
- Article
- Diagnosis of Human Endemic Mycoses Caused by Thermally Dimorphic Fungi: From Classical to Molecular Methods.Journal of fungi (Basel, Switzerland) · 2024Review
- Application of recombinase polymerase amplification with lateral flow assay to pathogen point-of-care diagnosis.Frontiers in cellular and infection microbiology · 2024Review
- Specific and sensitive loop-mediated isothermal amplification (LAMP) method for Madurella strains, eumycetoma filamentous fungi causative agent.PLoS neglected tropical diseases · 2023Article
- First report on mycetoma in Turkana County-North-western Kenya.PLoS neglected tropical diseases · 2023Article
- A scoping review of mycetoma profile in Egypt: revisiting the global endemicity map.Transactions of the Royal Society of Tropical Medicine and Hygiene · 2023Article
- Diagnostics to support mycetoma management-Development of two target product profiles.Tropical medicine & international health : TM & IH · 2022Article
- Article
- Madurella mycetomatis grains within a eumycetoma lesion are clonal.Medical mycology · 2022Article
- Mycetoma: Development of Diagnosis and Treatment.Journal of fungi (Basel, Switzerland) · 2022Review
- Direct detection of methicillin-resistant in Staphylococcus spp. in positive blood culture by isothermal recombinase polymerase amplification combined with lateral flow dipstick assay.World journal of microbiology & biotechnology · 2020Article
- The genus Madurella: Molecular identification and epidemiology in Sudan.PLoS neglected tropical diseases · 2020Article
- Draft Genome Sequences of Three Clinical Isolates of Madurella mycetomatis, the Major Cause of Black-Grain Mycetoma.Microbiology resource announcements · 2020Article
- Madurella real-time PCR, a novel approach for eumycetoma diagnosis.PLoS neglected tropical diseases · 2020Article
- Identification of Mycoses in Developing Countries.Journal of fungi (Basel, Switzerland) · 2019Review
- The Accuracy of Histopathological and Cytopathological Techniques in the Identification of the Mycetoma Causative Agents.PLoS neglected tropical diseases · 2019Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Appropriate diagnosis and treatment of eumycetoma may vary significantly depending on the causative agent. To date, the most common fungus causing mycetoma worldwide is Madurella mycetomatis. This species fails to express any recognizable morphological characteristics, and reliable identification can therefore only be achieved with the application of molecular techniques. Recombinase polymerase amplification (RPA) and loop-mediated isothermal amplification (LAMP) are proposed as alternatives to phenotypic methods. Species-specific primers were developed to target the ribosomal DNA (rDNA) internal transcribed spacer (ITS) region of M. mycetomatis. Both isothermal amplification techniques showed high specificity and sufficient sensitivity to amplify fungal DNA and proved to be appropriate for detection of M. mycetomatis. Diagnostic performance of the techniques was assessed in comparison to conventional PCR using biopsy specimens from eumycetoma patients. RPA is reliable and easy to operate and has the potential to be implemented in areas where mycetoma is endemic. The techniques may be expanded to detect fungal DNA from environmental samples.
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