Evidence map›Paper›PMID 26246398›Full record

ArticleCell and tissue research2015

CXCR4/CXCL12 signaling impacts enamel progenitor cell proliferation and motility in the dental stem cell niche.

Tamaki Yokohama-Tamaki, Keishi Otsu, Hidemitsu Harada, Shunichi Shibata, Nobuko Obara, Kazuharu Irie, Akiyoshi Taniguchi, Takashi Nagasawa, Kazunari Aoki, Steven R Caliari and 2 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell and tissue research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 48% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 5 citations in OpenAlex.

  1. Review
  2. Plasticity within the niche ensures the maintenance of aDevelopment (Cambridge, England) · 2018
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 6 institutions in 2 countries.

Tamaki Yokohama-TamakiDivision of Histology, Department of Oral Growth and Development, School of Dentistry, Health sciences University of Hokkaido, Hokkaido, Japan. ttamaki.mfa@tmd.ac.jp.
Keishi OtsuDivision of Oral Histology and Developmental Biology, School of Dentistry, Iwate Medical University, Iwate, Japan.
Hidemitsu HaradaDivision of Oral Histology and Developmental Biology, School of Dentistry, Iwate Medical University, Iwate, Japan.
Shunichi ShibataMaxillofacial Anatomy, Department of Maxillofacial Biology, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan.
Nobuko ObaraDivision of Histology, Department of Oral Growth and Development, School of Dentistry, Health sciences University of Hokkaido, Hokkaido, Japan.
Kazuharu IrieDivision of Histology, Department of Oral Growth and Development, School of Dentistry, Health sciences University of Hokkaido, Hokkaido, Japan.
Akiyoshi TaniguchiBiomaterials Center, National Institute for Materials Science, Ibaraki, Japan.
Takashi NagasawaDepartment of Immunobiology and Hematology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan.
Kazunari AokiDepartment of Immunobiology and Hematology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan.
Steven R CaliariDepartment of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, IL, USA.
Daniel W WeisgerberDepartment of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, IL, USA.
Brendan A C HarleyDepartment of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, IL, USA. bharley@illinois.edu.
University of Illinois Urbana-Champaign · USHealth Sciences University of Hokkaido · JPIwate Medical University · JPKyoto University · JPTokyo Medical and Dental University · JPNational Institute for Materials Science · JP

Funding

Training Program in Chemistry-Interface with BiologyT32GM070421 · NIGMS · UNIVERSITY OF ILLINOIS URBANA-CHAMPAIGN · PI HERGENROTHER, PAUL · 2005 to 2019
$5.4M
NIGMS NIH HHS T32 GM070421NIGMS NIH HHS T32GM070421
6 · The paper itself

Abstract

Dental stem cells are located at the proximal ends of rodent incisors. These stem cells reside in the dental epithelial stem cell niche, termed the apical bud. We focused on identifying critical features of a chemotactic signal in the niche. Here, we report that CXCR4/CXCL12 signaling impacts enamel progenitor cell proliferation and motility in dental stem cell niche cells. We report cells in the apical bud express CXCR4 mRNA at high levels while expression is restricted in the basal epithelium (BE) and transit-amplifying (TA) cell regions. Furthermore, the CXCL12 ligand is present in mesenchymal cells adjacent to the apical bud. We then performed gain- and loss-of-function analyses to better elucidate the role of CXCR4 and CXCL12. CXCR4-deficient mice contain epithelial cell aggregates, while cell proliferation in mutant incisors was also significantly reduced. We demonstrate in vitro that dental epithelial cells migrate toward sources of CXCL12, whereas knocking down CXCR4 impaired motility and resulted in formation of dense cell colonies. These results suggest that CXCR4 expression may be critical for activation of enamel progenitor cell division and that CXCR4/CXCL12 signaling may control movement of epithelial progenitors from the dental stem cell niche.

Indexed as

Cell MovementSignal TransductionStem Cell NicheAnimalsCell AggregationCell LineCell ProliferationCell ShapeChemokine CXCL12Dental EnamelEpithelial CellsGene Expression RegulationGene Knockdown TechniquesIncisorMice, KnockoutMutationChemokine CXCL12Receptors, CXCR4RNA, MessengerCXCR4/CXCL12 signalDental stem cell nicheMigrationProliferationTooth

Identifiers

PMID26246398
PMCPMC4679681
OpenAlexW1526855904

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.