ArticleScientific reports2015
High-throughput sequencing enhanced phage display enables the identification of patient-specific epitope motifs in serum.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed.
- Serum-antibody Profiling of H3N2-infected Ferrets Using a Combinatorial Phage-display Random Peptide Library.Journal of molecular biology · 2026Article
- Identification and Expression of Immunogenic Mimotopes ofVaccines · 2026Article
- DECODE enables high-throughput mapping of antibody epitopes at single amino acid resolution.PLoS biology · 2025Article
- Pollen-Food Allergy Syndrome: From Food Avoidance to Deciphering the Potential Cross-Reactivity between Pru p 3 and Ole e 7.Nutrients · 2024Article
- Phage Display Panning on Silica: Optimization of Elution Conditions for Selection of Strong Binders.Langmuir : the ACS journal of surfaces and colloids · 2024Article
- In vivo phage display identifies novel peptides for cardiac targeting.Scientific reports · 2024Article
- Genetically engineered bacteriophages as novel nanomaterials: applications beyond antimicrobial agents.Frontiers in bioengineering and biotechnology · 2024Review
- Review
- A Novel Strategy for Screening Tumor-Specific Variable Domain of Heavy-Chain Antibodies.International journal of molecular sciences · 2023Article
- Depth of Sequencing Plays a Determining Role in the Characterization of Phage Display Peptide Libraries by NGS.International journal of molecular sciences · 2023Article
- Identification of heptapeptides targeting a lethal bacterial strain in septic mice through an integrative approach.Signal transduction and targeted therapy · 2022Article
- Small Bispecific Affinity Proteins for Simultaneous Target Binding and Albumin-Associated Half-Life Extension.Molecular pharmaceutics · 2021Article
- Review
- Food-Derived Opioid Peptides in Human Health: A Review.International journal of molecular sciences · 2020Review
- Repertoire-scale determination of class II MHC peptide binding via yeast display improves antigen prediction.Nature communications · 2020Article
- Multifaceted antibodies development against synthetic α-dystroglycan mucin glycopeptide as promising tools for dystroglycanopathies diagnostic.Glycoconjugate journal · 2020Article
- Article
- Single Domain Antibodies Targeting Receptor Binding Pockets of NadA Restrain Adhesion ofFrontiers in molecular biosciences · 2020Article
- Identification of Serum Biomarkers for Systemic Lupus Erythematosus Using a Library of Phage Displayed Random Peptides and Deep Sequencing.Molecular & cellular proteomics : MCP · 2019Article
- Bacteriophage-based biomaterials for tissue regeneration.Advanced drug delivery reviews · 2019Review
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Authors and funding
13 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phage display is a prominent screening technique with a multitude of applications including therapeutic antibody development and mapping of antigen epitopes. In this study, phages were selected based on their interaction with patient serum and exhaustively characterised by high-throughput sequencing. A bioinformatics approach was developed in order to identify peptide motifs of interest based on clustering and contrasting to control samples. Comparison of patient and control samples confirmed a major issue in phage display, namely the selection of unspecific peptides. The potential of the bioinformatic approach was demonstrated by identifying epitopes of a prominent peanut allergen, Ara h 1, in sera from patients with severe peanut allergy. The identified epitopes were confirmed by high-density peptide micro-arrays. The present study demonstrates that high-throughput sequencing can empower phage display by (i) enabling the analysis of complex biological samples, (ii) circumventing the traditional laborious picking and functional testing of individual phage clones and (iii) reducing the number of selection rounds.
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