ArticleScientific reports2015
Ultrafast excited-state dynamics and fluorescence deactivation of near-infrared fluorescent proteins engineered from bacteriophytochromes.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Rapid Biodistribution of Fluorescent Outer-Membrane Vesicles from the Intestine to Distant Organs via the Blood in Mice.International journal of molecular sciences · 2024Article
- Blue-shift photoconversion of near-infrared fluorescent proteins for labeling and tracking in living cells and organisms.Nature communications · 2023Article
- Protein-chromophore interactions controlling photoisomerization in red/green cyanobacteriochromes.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2022Article
- Influence of the Environment on Shaping the Absorption of Monomeric Infrared Fluorescent Proteins.The journal of physical chemistry. B · 2021Article
- Choosing Fluorescent Probes and Labeling Systems.Methods in molecular biology (Clifton, N.J.) · 2021Article
- The Origin of Ultrafast Multiphasic Dynamics in Photoisomerization of Bacteriophytochrome.The journal of physical chemistry letters · 2020Article
- Bacterial Phytochromes, Cyanobacteriochromes and Allophycocyanins as a Source of Near-Infrared Fluorescent Probes.International journal of molecular sciences · 2017Review
- Designing brighter near-infrared fluorescent proteins: insights from structural and biochemical studies.Chemical science · 2017Article
- Polarization-controlled optimal scatter suppression in transient absorption spectroscopy.Scientific reports · 2017Article
- Bright blue-shifted fluorescent proteins with Cys in the GAF domain engineered from bacterial phytochromes: fluorescence mechanisms and excited-state dynamics.Scientific reports · 2016Article
- The role of local and remote amino acid substitutions for optimizing fluorescence in bacteriophytochromes: A case study on iRFP.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Near-infrared fluorescent proteins, iRFPs, are recently developed genetically encoded fluorescent probes for deep-tissue in vivo imaging. Their functions depend on the corresponding fluorescence efficiencies and electronic excited state properties. Here we report the electronic excited state deactivation dynamics of the most red-shifted iRFPs: iRFP702, iRFP713 and iRFP720. Complementary measurements by ultrafast broadband fluorescence and absorption spectroscopy show that single exponential decays of the excited state with 600~700 ps dominate in all three iRFPs, while photoinduced isomerization was completely inhibited. Significant kinetic isotope effects (KIE) were observed with a factor of ~1.8 in D2O, and are interpreted in terms of an excited-state proton transfer (ESPT) process that deactivates the excited state in competition with fluorescence and chromophore mobility. On this basis, new approaches for rational molecular engineering may be applied to iRFPs to improve their fluorescence.
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