ArticleVirology journal2015
Characterization of emerging Newcastle disease virus isolates in China.
Article in Virology journal, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 33 citations in OpenAlex.
- Newcastle Disease Virus Fusion and Haemagglutinin-Neuraminidase Gene Divergence: Implications for Vaccines.Veterinary sciences · 2026Article
- Generation, characterization and epitope mapping of monoclonal antibody 4H3 specific to the HN protein of pigeon paramyxovirus type 1 (PPMV-1).Frontiers in microbiology · 2026Article
- Molecular characterization and genotyping of Newcastle disease viruses using whole-genome sequencing from poultry farms in parts of Amhara Regions, and central Ethiopia.Virology journal · 2025Article
- Subgenotype VII.1.1 Newcastle Disease Virus Evolution and Spread in the Russian Federation in 2019-2023.Viruses · 2025Article
- Article
- Concurrent pigeon paramyxovirus-1 andEmerging microbes & infections · 2022Article
- Screening and identification of nucleocapsid protein-nanobodies that inhibited Newcastle disease virus replication in DF-1 cells.Frontiers in microbiology · 2022Article
- Phylogenetic relationship and genotype variation of six Newcastle disease viruses isolated from duck in Indonesia.Veterinary world · 2021Article
- Insights into Genomic Epidemiology, Evolution, and Transmission Dynamics of Genotype VII of Class II Newcastle Disease Virus in China.Pathogens (Basel, Switzerland) · 2020Article
- Article
- Fenobody and RANbody-based sandwich enzyme-linked immunosorbent assay to detect Newcastle disease virus.Journal of nanobiotechnology · 2020Article
- Efficacy of inactivated velogenic Newcastle disease virus genotype VII vaccine in broiler chickens.Veterinary research forum : an international quarterly journal · 2020Article
- Viral infection detection using metagenomics technology in six poultry farms of eastern China.PloS one · 2019Article
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundNewcastle disease (ND) is a devastating worldwide disease of poultry characterized by increased respiration, circulatory disturbances, hemorrhagic enteritis, and nervous signs. Sequence analysis shows several amino acid residue substitutions at neutralizing epitopes on the F and HN proteins of recent Shaanxi strains. Both Cross protection and cross serum neutralization tests revealed that the traditional vaccine strains were unable to provide full protection for the flocks.
methodsTo better understand the epidemiology of Newcastle disease outbreak, a portion of the F gene and the full-length HN gene were amplified from Shaanxi isolates by reverse transcription-polymerase chain reaction (RT-PCR) and then conducted sequence and phylogenetic analyzes. In pathogenicity analysis, both high intra-cerebral pathogenicity index (ICPI) and mean death time (MDT) tests of chicken embryo were carried out. Furthermore, a cross-protection experiment in which specific-pathogen-free chickens vaccinated with a LaSota vaccine strain were challenged by the recent Shaanxi strain was also performed.
resultsNine Newcastle disease (ND) virus (NDV) isolates which were recovered from ND outbreaks in chicken flocks in China were genotypically and pathotypically characterized. Amino acid sequence analysis revealed that all the recent Shaanxi-isolated NDVs have (112)R-R-Q-K-R-F(117) for the C-terminus of the F2 protein and exhibit high ICPI and MDT of chicken embryos, suggesting that they were all classified as velogenic type of NDVs. Phylogenetic analysis of these isolates showed that they belong to subgenotype VIId that have been implicated in the recent outbreaks in northwestern China. The percentage of amino acid sequence identity of F protein between recent Shaanxi stains and five vaccine strains was in the range of 81.9 %-88.1 %, while the percentage of amino acid sequence identity of HN protein between recent Shaanxi strains and vaccine strains was in the range of 87.4 %-91.2 %. Furthermore, a number of amino acid residue substitutions at neutralizing epitopes on the F and HN proteins of these isolates were observed, which may lead to the change of antibody recognition and neutralization capacity. A cross-protection experiment indicated that specific-pathogen-free chickens vaccinated with a LaSota vaccine strain was not capable of providing full protection for the flocks that were challenged by the recent Shaanxi strain.
conclusionsTaken together, our findings reveal that recent Shannxi NDVstrains exhibit antigenic variations that could be responsible for recent outbreaks of NDVs in northwestern China.
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