ArticleMicrobial cell factories2015
Genome shuffling of the nonconventional yeast Pichia anomala for improved sugar alcohol production.
Article in Microbial cell factories, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Isolation, screening, and characterization of the newly isolated osmotolerant yeast Wickerhamomyces anomalus BKK11-4 for the coproduction of glycerol and arabitol.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024Article
- Characterization of yeast mutant strains for starter culture in Arabica coffee fermentation.Scientific reports · 2024Article
- Modulating DNA Repair Pathways to Diversify Genomic Alterations in Saccharomyces cerevisiae.Microbiology spectrum · 2022Article
- Application of Non-Thermal Plasma to Fungal Resources.Journal of fungi (Basel, Switzerland) · 2022Review
- Inference of Regulatory System for TAG Biosynthesis inBioengineering (Basel, Switzerland) · 2020Article
- An innovative protein expression system using RNA polymerase I for large-scale screening of high-nucleic-acid content Saccharomyces cerevisiae strains.Microbial biotechnology · 2020Article
- Applications and research advance of genome shuffling for industrial microbial strains improvement.World journal of microbiology & biotechnology · 2020Review
- Article
- Improvement ofFood science and biotechnology · 2018Article
- Improving Iturin A Production ofFrontiers in microbiology · 2018Article
- Improving isopropanol tolerance and production of Clostridium beijerinckii DSM 6423 by random mutagenesis and genome shuffling.Applied microbiology and biotechnology · 2016Article
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Authors and funding
7 authors.
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Abstract
backgroundSugar alcohols have been widely applied in the fields of food and medicine owing to their unique properties. Compared to chemical production, microbial production of sugar alcohols has become attractive because of its environmentally friendly and sustainable characteristics. Our previous study identified the nonconventional yeast Pichia anomala TIB-x229 as a potential producer of sugar alcohols from glucose. To further improve strain performance, we combined genome shuffling with optimized high throughput screening methods for the directed improvement of nonconventional yeast and complex phenotypes.
resultsTo accelerate strain improvement, a practical genome shuffling procedure was developed and successfully applied in the nonconventional yeast P. anomala to increase sugar alcohol production. Through two rounds of genome shuffling, an improved P. anomala isolate GS2-3 could produce 47.1 g/L total sugar alcohols from 100 g/L glucose, which was 32.3% higher than the original strain. In this process, a simple and accurate colorimetric assay was optimized and used for high throughput screening of sugar alcohol-producing strains. Moreover, a fluorescence-activated cell sorting method was developed to efficiently screen protoplast fusions for genome shuffling of nonconventional yeast.
conclusionAn efficient genome shuffling procedure was developed and applied to enhance the sugar alcohol production of the nonconventional yeast P. anomala. Our results provide a general platform for strain improvement of polyol-producing microorganisms or nonconventional microorganisms in the future.
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