ArticlePloS one2015
Prenatal Exposure to DEHP Affects Spermatogenesis and Sperm DNA Methylation in a Strain-Dependent Manner.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed, 1 synthesis or guideline pooled it, 56 citations in OpenAlex.
- A systematic review on the role of paternal factors in human placental development, function, and pregnancy-related disorders.Journal of assisted reproduction and genetics · 2025Pooled it
- lncRNA-operated stem cell fate control: RPS3 mediates the in situ Gm16751-Zhx3 interactome to program spermatogonial stem cell differentiation.Cell & bioscience · 2026Article
- Methods for Investigating the Sperm Epigenome.Advances in experimental medicine and biology · 2026Review
- Sperm epigenetics and sperm RNAs as drivers of male infertility: truth or myth?Molecular and cellular biochemistry · 2025Review
- Phthalates and epigenetics: An emerging public health concern.Current research in toxicology · 2025Review
- Role of sleep in asthenospermia induced by di (2-ethyl-hexyl) phthalate.Environmental science and pollution research international · 2024Article
- Loss of Nuclear/DNA Integrity in Mouse Epididymal Spermatozoa after Short-Term Exposure to Low Doses of Dibutyl Phthalate or Bisphenol AF and Its Mitigation by Oral Antioxidant Supplementation.Antioxidants (Basel, Switzerland) · 2023Article
- Microplastics exposure: implications for human fertility, pregnancy and child health.Frontiers in endocrinology · 2023Review
- Inheritance of environment-induced phenotypic changes through epigenetic mechanisms.Environmental epigenetics · 2023Review
- Identification of spermatogenesis-associated changes in DNA methylation induced by maternal exposure to chemicals in male germ cells.STAR protocols · 2022Article
- Prenatal exposure to the phthalate DEHP impacts reproduction-related gene expression in the pituitary.Reproductive toxicology (Elmsford, N.Y.) · 2022Article
- Epigenetic transgenerational inheritance, gametogenesis and germline development†.Biology of reproduction · 2021Review
- Epi-mutations for spermatogenic defects by maternal exposure to di(2-ethylhexyl) phthalate.eLife · 2021Article
- Transgenerational Effects of Di(2-Ethylhexyl) Phthalate on Anogenital Distance, Sperm Functions and DNA Methylation in Rat Offspring.International journal of molecular sciences · 2021Article
- Perinatal DEHP exposure induces sex- and tissue-specific DNA methylation changes in both juvenile and adult mice.Environmental epigenetics · 2021Article
- Environmental Impact on Male (In)Fertility via Epigenetic Route.Journal of clinical medicine · 2020Review
- Endocrine-disrupting chemicals and male reproductive health.Reproductive medicine and biology · 2020Review
- Phthalate Exposure and Long-Term Epigenomic Consequences: A Review.Frontiers in genetics · 2020Review
- Androgenic/antiandrogenic activities of PAEs determined by a novel AR-mediated reporter gene assay based on LLC-MK2 cells.Toxicology research · 2019Article
- The Increase of ROS Caused by the Interference of DEHP with JNK/p38/p53 Pathway as the Reason for Hepatotoxicity.International journal of environmental research and public health · 2019Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Di-(2-ethylhexyl)phtalate (DEHP) is a plasticizer with endocrine disrupting properties found ubiquitously in the environment and altering reproduction in rodents. Here we investigated the impact of prenatal exposure to DEHP on spermatogenesis and DNA sperm methylation in two distinct, selected, and sequenced mice strains. FVB/N and C57BL/6J mice were orally exposed to 300 mg/kg/day of DEHP from gestation day 9 to 19. Prenatal DEHP exposure significantly decreased spermatogenesis in C57BL/6J (fold-change = 0.6, p-value = 8.7*10-4), but not in FVB/N (fold-change = 1, p-value = 0.9). The number of differentially methylated regions (DMRs) by DEHP-exposure across the entire genome showed increased hyper- and decreased hypo-methylation in C57BL/6J compared to FVB/N. At the promoter level, three important subsets of genes were massively affected. Promoters of vomeronasal and olfactory receptors coding genes globally followed the same trend, more pronounced in the C57BL/6J strain, of being hyper-methylated in DEHP related conditions. In contrast, a large set of micro-RNAs were hypo-methylated, with a trend more pronounced in the FVB/N strain. We additionally analyze both the presence of functional genetic variations within genes that were associated with the detected DMRs and that could be involved in spermatogenesis, and DMRs related with the DEHP exposure that affected both strains in an opposite manner. The major finding in this study indicates that prenatal exposure to DEHP can decrease spermatogenesis in a strain-dependent manner and affects sperm DNA methylation in promoters of large sets of genes putatively involved in both sperm chemotaxis and post-transcriptional regulatory mechanisms.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.