ArticlePloS one2015
Virulence Characterization of Salmonella enterica by a New Microarray: Detection and Evaluation of the Cytolethal Distending Toxin Gene Activity in the Unusual Host S. Typhimurium.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 46 citations in OpenAlex.
- Genetic insights into antimicrobial resistance and virulence characteristics of Salmonella enterica isolated from Nile tilapia sourced from retail markets in Thailand.BMC microbiology · 2025Article
- Pathogenicity assessment and whole-genome sequencing ofFrontiers in veterinary science · 2025Article
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- Genomic Diversity, Antimicrobial Resistance, Plasmidome, and Virulence Profiles ofAntibiotics (Basel, Switzerland) · 2023Article
- Review
- Recent Advances on Peptide-Based Biosensors and Electronic Noses for Foodborne Pathogen Detection.Biosensors · 2023Review
- Pathogen genomics and phage-based solutions for accurately identifying and controllingFrontiers in microbiology · 2023Article
- Genotypic virulence profiles and associations in Salmonella isolated from meat samples in wet markets and abattoirs of Metro Manila, Philippines.BMC microbiology · 2022Article
- Article
- Harmonisation of in-silico next-generation sequencing based methods for diagnostics and surveillance.Scientific reports · 2022Article
- Proteomic Characterization of the Oral PathogenPathogens (Basel, Switzerland) · 2022Article
- Recent Advancements in the Technologies Detecting Food Spoiling Agents.Journal of functional biomaterials · 2021Review
- Whole-Genome Sequencing ofFrontiers in microbiology · 2021Article
- Identification of Genetic Features for Attenuation of TwoFrontiers in veterinary science · 2019Article
- Whole genome sequencing reveals widespread distribution of typhoidal toxin genes and VirB/D4 plasmids in bovine-associated nontyphoidal Salmonella.Scientific reports · 2018Article
- Campylobacteriosis, Salmonellosis, Yersiniosis, and Listeriosis as Zoonotic Foodborne Diseases: A Review.International journal of environmental research and public health · 2018Review
- Virulent nontyphoidal Salmonella producing CTX-M and CMY-2 β-lactamases from livestock, food and human infection, Brazil.Virulence · 2018Article
- Polyphyletic Nature ofFrontiers in microbiology · 2018Article
- Biofilm Producing Salmonella Typhi: Chronic Colonization and Development of Gallbladder Cancer.International journal of molecular sciences · 2017Review
- Microarray Evaluation of Antimicrobial Resistance and Virulence of Escherichia coli Isolates from Portuguese Poultry.Antibiotics (Basel, Switzerland) · 2016Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Salmonella enterica is a zoonotic foodborne pathogen that causes acute gastroenteritis in humans. We assessed the virulence potential of one-hundred and six Salmonella strains isolated from food animals and products. A high through-put virulence genes microarray demonstrated Salmonella Pathogenicity Islands (SPI) and adherence genes were highly conserved, while prophages and virulence plasmid genes were variably present. Isolates were grouped by serotype, and virulence plasmids separated S. Typhimurium in two clusters. Atypical microarray results lead to whole genome sequencing (WGS) of S. Infantis Sal147, which identified deletion of thirty-eight SPI-1 genes. Sal147 was unable to invade HeLa cells and showed reduced mortality in Galleria mellonella infection model, in comparison to a SPI-1 harbouring S. Infantis. Microarray and WGS of S. Typhimurium Sal199, established for the first time in S. Typhimurium presence of cdtB and other Typhi-related genes. Characterization of Sal199 showed cdtB genes were upstream of transposase IS911, and co-expressed with other Typhi-related genes. Cell cycle arrest, cytoplasmic distension, and nuclear enlargement were detected in HeLa cells infected by Sal199, but not with S. Typhimurium LT2. Increased mortality of Galleria was detected on infection with Sal199 compared to LT2. Thus, Salmonella isolates were rapidly characterized using a high through-put microarray; helping to identify unusual virulence features which were corroborated by further characterisation. This work demonstrates that the use of suitable screening methods for Salmonella virulence can help assess the potential risk associated with certain Salmonella to humans. Incorporation of such methodology into surveillance could help reduce the risk of emergence of epidemic Salmonella strains.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.