ArticleACS applied materials & interfaces2015
Protective Polymer Coatings for High-Throughput, High-Purity Cellular Isolation.
Article in ACS applied materials & interfaces, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed, 24 citations in OpenAlex.
- Conformal encapsulation of mammalian stem cells using modified hyaluronic acid.Journal of materials chemistry. B · 2024Article
- Substituent Effects of Fluorescein on Photoredox Initiating Performance under Visible Light.ACS omega · 2023Article
- Increased Retention of Cardiac Cells to a Glass Substrate through Streptavidin-Biotin Affinity.ACS omega · 2021Article
- Increased yield of gelatin coated therapeutic cells through cholesterol insertion.Journal of biomedical materials research. Part A · 2021Article
- Cell Death Persists in Rapid Extrusion of Lysis-Resistant Coated Cardiac Myoblasts.Bioprinting (Amsterdam, Netherlands) · 2020Article
- Gelatin Based Polymer Cell Coating Improves Bone Marrow-Derived Cell Retention in the Heart after Myocardial Infarction.Stem cell reviews and reports · 2019Article
- Coatings on mammalian cells: interfacing cells with their environment.Journal of biological engineering · 2019Review
- Comparison of eosin and fluorescein conjugates for the photoinitiation of cell-compatible polymer coatings.PloS one · 2018Article
- Hydrogel Patches on Live Cells through Surface-Mediated Polymerization.Langmuir : the ACS journal of surfaces and colloids · 2017Article
- Polymeric Biomaterials: Diverse Functions Enabled by Advances in Macromolecular Chemistry.Macromolecules · 2017Article
- The Role of Surface Receptor Density in Surface-Initiated Polymerizations for Cancer Cell Isolation.Langmuir : the ACS journal of surfaces and colloids · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Cell-based therapies are emerging as the next frontier of medicine, offering a plausible path forward in the treatment of many devastating diseases. Critically, current methods for antigen positive cell sorting lack a high throughput method for delivering ultrahigh purity populations, prohibiting the application of some cell-based therapies to widespread diseases. Here we show the first use of targeted, protective polymer coatings on cells for the high speed enrichment of cells. Individual, antigen-positive cells are coated with a biocompatible hydrogel which protects the cells from a surfactant solution, while uncoated cells are immediately lysed. After lysis, the polymer coating is removed through orthogonal photochemistry, and the isolate has >50% yield of viable cells and these cells proliferate at rates comparable to control cells. Minority cell populations are enriched from erythrocyte-depleted blood to >99% purity, whereas the entire batch process requires 1 h and <$2000 in equipment. Batch scale-up is only contingent on irradiation area for the coating photopolymerization, as surfactant-based lysis can be easily achieved on any scale.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.