ArticlePeerJ2015
From promise to practice: pairing non-invasive sampling with genomics in conservation.
Article in PeerJ, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 75 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
75 citing papers in PubMed, 188 citations in OpenAlex.
- Comparative population genomics reveal stark differences in the genomic structure and drivers of isolation for two sky island bumble bees.Proceedings. Biological sciences · 2026Article
- Population Genomics Approaches Identify a Cryptic, Emerging Generalist Pest Complex.Ecology and evolution · 2026Article
- Umbilical Cord: A Non-Invasive DNA Source for Sea Turtle Genomics.Molecular ecology resources · 2026Article
- Maximizing the Quality of Non-Invasive Samples for Conservation Genetics Using Targeted Next-Generation Sequencing: A Comparison of Fecal DNA Preservation Methodologies.Ecology and evolution · 2026Article
- Assessment of High Throughput Sequencing Quality of Host DNA Enriched From Faeces: A Case From Captive Tiger.Molecular ecology resources · 2026Article
- Mitochondrial structure despite nuclear panmixia: sex-specific dispersal dictates population structure in sperm whales.Heredity · 2026Article
- Landscape Genomics and Evolutionary History ofEvolutionary applications · 2026Article
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- Discovery of Genetically Distinct, Sympatric Coral Lineages With Temporal but Not Gametic Reproductive Isolation.Molecular ecology · 2025Article
- Hybridization as driving force for cryptic species diversity in the Caribbean coral genus Madracis.Scientific reports · 2025Article
- Genomic Diversity and Structure ofPlants (Basel, Switzerland) · 2025Article
- Age Is Just a Number: No Impacts of Scat Ageing on Single Nucleotide Polymorphism Genotyping Using a Target Capture Approach.Ecology and evolution · 2025Article
- Pollination niche shifts in sympatric globular cactiPlant diversity · 2025Article
- The Genome of Lolium multiflorum Reveals the Genetic Architecture of Paraquat Resistance.Molecular ecology · 2025Article
- Depth-structured lineages in the coral Stylophora pistillata of the Northern Red Sea.npj biodiversity · 2025Article
- Genomic structure and functional trait variation are decoupled across the Atacama-Patagonia arid gradient in the Chilean wineberry.Frontiers in plant science · 2025Article
- A Tale of Two Lakes: Divergent Evolutionary Trajectories of TwoEcology and evolution · 2024Article
- Genetic mechanisms of axial patterning inEvolution letters · 2024Article
15 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Conservation genomics has become an increasingly popular term, yet it remains unclear whether the non-invasive sampling that is essential for many conservation-related studies is compatible with the minimum requirements for harnessing next-generation sequencing technologies. Here, we evaluated the feasibility of using genotyping-by-sequencing of non-invasively collected hair samples to simultaneously identify and genotype single nucleotide polymorphisms (SNPs) in a climate-sensitive mammal, the American pika (Ochotona princeps). We identified and genotyped 3,803 high-confidence SNPs across eight sites distributed along two elevational transects using starting DNA amounts as low as 1 ng. Fifty-five outlier loci were detected as candidate gene regions under divergent selection, constituting potential targets for future validation. Genome-wide estimates of gene diversity significantly and positively correlated with elevation across both transects, with all low elevation sites exhibiting significant heterozygote deficit likely due to inbreeding. More broadly, our results highlight a range of issues that must be considered when pairing genomic data collection with non-invasive sampling, particularly related to field sampling protocols for minimizing exogenous DNA, data collection strategies and quality control steps for enhancing target organism yield, and analytical approaches for maximizing cost-effectiveness and information content of recovered genomic data.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.