Evidence map›Paper›PMID 26243620›Full record

ArticlePlant physiology2015

Distinct Cellular Locations of Carbonic Anhydrases Mediate Carbon Dioxide Control of Stomatal Movements.

Honghong Hu, Wouter-Jan Rappel, Rossana Occhipinti, Amber Ries, Maik Böhmer, Lei You, Chuanlei Xiao, Cawas B Engineer, Walter F Boron, Julian I Schroeder

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Plant physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.

0numbers the graph read from it
0cells of the map it votes in
45citing papers in PubMed
4.7field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

45 citing papers in PubMed, 107 citations in OpenAlex.

  1. Review
  2. Review
  3. TheGenome research · 2025
    Article
  4. Article
  5. Article
  6. COPhilosophical transactions of the Royal Society of London. Series B, Biological sciences · 2025
    Review
  7. Article
  8. Article
  9. Article
  10. Article
  11. Identification of QTLs for symbiotic nitrogen fixation and related traits in a soybean recombinant inbred line population.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024
    Article
  12. Carbonic anhydrases in the cell wall and plasma membrane ofFrontiers in molecular biosciences · 2024
    Article
  13. Article
  14. Review
  15. Elevated COThe New phytologist · 2022
    Article
  16. Article
  17. Jasmonic acid and salicylic acid play minor roles in stomatal regulation by COThe Plant journal : for cell and molecular biology · 2021
    Article
  18. Absence of carbonic anhydrase in chloroplasts affects CProceedings of the National Academy of Sciences of the United States of America · 2021
    Article
  19. Review
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 3 countries.

Honghong HuCollege of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China (H.H., L.Y., C.X.);Division of Biological Sciences, Cell and Developmental Biology Section (H.H., A.R., M.B., C.B.E., J.I.S.) andPhysics Department (W.-J.R.), University of California, San Diego, La Jolla, California 92093-0116; andDepartment of Physiology and Biophysics (R.O., W.F.B.) andDepartment of Medicine (W.F.B.), Case Western Reserve University School of Medicine, Cleveland, Ohio 44106 huhh@mail.hzau.edu.cn jischroeder@ucsd.edu.ORCID http://orcid.org/0000-0003-0538-6646
Wouter-Jan RappelCollege of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China (H.H., L.Y., C.X.);Division of Biological Sciences, Cell and Developmental Biology Section (H.H., A.R., M.B., C.B.E., J.I.S.) andPhysics Department (W.-J.R.), University of California, San Diego, La Jolla, California 92093-0116; andDepartment of Physiology and Biophysics (R.O., W.F.B.) andDepartment of Medicine (W.F.B.), Case Western Reserve University School of Medicine, Cleveland, Ohio 44106.
Rossana OcchipintiCollege of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China (H.H., L.Y., C.X.);Division of Biological Sciences, Cell and Developmental Biology Section (H.H., A.R., M.B., C.B.E., J.I.S.) andPhysics Department (W.-J.R.), University of California, San Diego, La Jolla, California 92093-0116; andDepartment of Physiology and Biophysics (R.O., W.F.B.) andDepartment of Medicine (W.F.B.), Case Western Reserve University School of Medicine, Cleveland, Ohio 44106.
Amber RiesCollege of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China (H.H., L.Y., C.X.);Division of Biological Sciences, Cell and Developmental Biology Section (H.H., A.R., M.B., C.B.E., J.I.S.) andPhysics Department (W.-J.R.), University of California, San Diego, La Jolla, California 92093-0116; andDepartment of Physiology and Biophysics (R.O., W.F.B.) andDepartment of Medicine (W.F.B.), Case Western Reserve University School of Medicine, Cleveland, Ohio 44106.
Maik BöhmerCollege of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China (H.H., L.Y., C.X.);Division of Biological Sciences, Cell and Developmental Biology Section (H.H., A.R., M.B., C.B.E., J.I.S.) andPhysics Department (W.-J.R.), University of California, San Diego, La Jolla, California 92093-0116; andDepartment of Physiology and Biophysics (R.O., W.F.B.) andDepartment of Medicine (W.F.B.), Case Western Reserve University School of Medicine, Cleveland, Ohio 44106.ORCID http://orcid.org/0000-0001-6675-273X
Lei YouCollege of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China (H.H., L.Y., C.X.);Division of Biological Sciences, Cell and Developmental Biology Section (H.H., A.R., M.B., C.B.E., J.I.S.) andPhysics Department (W.-J.R.), University of California, San Diego, La Jolla, California 92093-0116; andDepartment of Physiology and Biophysics (R.O., W.F.B.) andDepartment of Medicine (W.F.B.), Case Western Reserve University School of Medicine, Cleveland, Ohio 44106.
Chuanlei XiaoCollege of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China (H.H., L.Y., C.X.);Division of Biological Sciences, Cell and Developmental Biology Section (H.H., A.R., M.B., C.B.E., J.I.S.) andPhysics Department (W.-J.R.), University of California, San Diego, La Jolla, California 92093-0116; andDepartment of Physiology and Biophysics (R.O., W.F.B.) andDepartment of Medicine (W.F.B.), Case Western Reserve University School of Medicine, Cleveland, Ohio 44106.
Cawas B EngineerCollege of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China (H.H., L.Y., C.X.);Division of Biological Sciences, Cell and Developmental Biology Section (H.H., A.R., M.B., C.B.E., J.I.S.) andPhysics Department (W.-J.R.), University of California, San Diego, La Jolla, California 92093-0116; andDepartment of Physiology and Biophysics (R.O., W.F.B.) andDepartment of Medicine (W.F.B.), Case Western Reserve University School of Medicine, Cleveland, Ohio 44106.
Walter F BoronCollege of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China (H.H., L.Y., C.X.);Division of Biological Sciences, Cell and Developmental Biology Section (H.H., A.R., M.B., C.B.E., J.I.S.) andPhysics Department (W.-J.R.), University of California, San Diego, La Jolla, California 92093-0116; andDepartment of Physiology and Biophysics (R.O., W.F.B.) andDepartment of Medicine (W.F.B.), Case Western Reserve University School of Medicine, Cleveland, Ohio 44106.
Julian I SchroederCollege of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China (H.H., L.Y., C.X.);Division of Biological Sciences, Cell and Developmental Biology Section (H.H., A.R., M.B., C.B.E., J.I.S.) andPhysics Department (W.-J.R.), University of California, San Diego, La Jolla, California 92093-0116; andDepartment of Physiology and Biophysics (R.O., W.F.B.) andDepartment of Medicine (W.F.B.), Case Western Reserve University School of Medicine, Cleveland, Ohio 44106 huhh@mail.hzau.edu.cn jischroeder@ucsd.edu.
University of California San Diego · USHuazhong Agricultural University · CN

Funding

SYNTHETIC SYSTEMSP50GM085764 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI HASTY, JEFF M, IDEKER, TREY · 2010 to 2018
$23.3M
STRESS HORMONE SIGNAL TRANSDUCTION IN ARABIDOPSISR01GM060396 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI SCHROEDER, JULIAN I · 2000 to 2023
$7.4M
Multi-scale modeling of gas transport through channels in living cellsU01GM111251 · NIGMS · CASE WESTERN RESERVE UNIVERSITY · PI BORON, WALTER F, SOMERSALO, ERKKI JAAKKO · 2015 to 2019
$2.9M
NIGMS NIH HHS GM060396NIGMS NIH HHS GM085764NIGMS NIH HHS P50 GM085764NIGMS NIH HHS R01 GM060396NIGMS NIH HHS U01 GM111251NIGMS NIH HHS U01-GM111251
6 · The paper itself

Abstract

Elevated carbon dioxide (CO2) in leaves closes stomatal apertures. Research has shown key functions of the β-carbonic anhydrases (βCA1 and βCA4) in rapid CO2-induced stomatal movements by catalytic transmission of the CO2 signal in guard cells. However, the underlying mechanisms remain unclear, because initial studies indicate that these Arabidopsis (Arabidopsis thaliana) βCAs are targeted to distinct intracellular compartments upon expression in tobacco (Nicotiana benthamiana) cells. Which cellular location of these enzymes plays a key role in native guard cells in CO2-regulated stomatal movements remains unknown. Here, we express fluorescently tagged CAs in guard cells of ca1ca4 double-mutant plants and show that the specific locations of βCA4 at the plasma membrane and βCA1 in native guard cell chloroplasts each can mediate rapid CO2 control of stomatal movements. Localization and complementation analyses using a mammalian αCAII-yellow fluorescent protein in guard cells further show that cytoplasmic localization is also sufficient to restore CO2 regulation of stomatal conductance. Mathematical modeling of cellular CO2 catalysis suggests that the dynamics of the intracellular HCO3 (-) concentration change in guard cells can be driven by plasma membrane and cytoplasmic localizations of CAs but not as clearly by chloroplast targeting. Moreover, modeling supports the notion that the intracellular HCO3 (-) concentration dynamics in guard cells are a key mechanism in mediating CO2-regulated stomatal movements but that an additional chloroplast role of CAs exists that has yet to be identified.

Indexed as

Amino Acid SequenceArabidopsisArabidopsis ProteinsBacterial ProteinsCarbon DioxideCarbonic AnhydrasesCell MembraneChloroplastsCytosolGreen Fluorescent ProteinsHumansLuminescent ProteinsModels, BiologicalMolecular Sequence DataNicotianaPlant CellsArabidopsis ProteinsBacterial ProteinsCA1 protein, ArabidopsisCA4 protein, ArabidopsisCarbon DioxideCarbonic AnhydrasesGreen Fluorescent ProteinsLuminescent Proteinsyellow fluorescent protein, Bacteria

Identifiers

PMID26243620
PMCPMC4587455
OpenAlexW2098620484

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.