Evidence map›Paper›PMID 26243474›Full record

ArticleJournal of cell science2015

Volume regulation and shape bifurcation in the cell nucleus.

Dong-Hwee Kim, Bo Li, Fangwei Si, Jude M Phillip, Denis Wirtz, Sean X Sun

Erratum issuedAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of cell science, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 62 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
62citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

62 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Article
  3. Article
  4. Actuation of Cell Layers in Three Dimensions.Advanced materials (Deerfield Beach, Fla.) · 2026
    Article
  5. Material-Induced Nuclear Deformation Controls Chromatin Architecture in Adipose Stem Cells.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026
    Article
  6. Article
  7. Article
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  11. Article
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  14. Cracks in tensile-contracting and tensile-dilating poroelastic materials.International journal of solids and structures · 2024
    Article
  15. Article
  16. Article
  17. Article
  18. Indentation induces instantaneous nuclear stiffening and unfolding of nuclear envelope wrinkles.Proceedings of the National Academy of Sciences of the United States of America · 2023
    Article
  19. Article
  20. Article

2 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

6 authors.

Dong-Hwee KimJohns Hopkins Physical Sciences-Oncology Center, Johns Hopkins University, Baltimore, MD 21218, USA Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD 21218, USA Disease Biophysics Group, Wyss Institute for Biologically Inspired Engineering, School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA, 4KU-KIST Graduate School of Converging Science and Technology, Korea University, Seoul, 02841, Republic of Korea.
Bo LiJohns Hopkins Physical Sciences-Oncology Center, Johns Hopkins University, Baltimore, MD 21218, USA Department of Mechanical Engineering, Johns Hopkins University, Baltimore, MD 21218, USA.
Fangwei SiJohns Hopkins Physical Sciences-Oncology Center, Johns Hopkins University, Baltimore, MD 21218, USA Department of Mechanical Engineering, Johns Hopkins University, Baltimore, MD 21218, USA.
Jude M PhillipJohns Hopkins Physical Sciences-Oncology Center, Johns Hopkins University, Baltimore, MD 21218, USA Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD 21218, USA.
Denis WirtzJohns Hopkins Physical Sciences-Oncology Center, Johns Hopkins University, Baltimore, MD 21218, USA Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD 21218, USA wirtz@jhu.edu ssun@jhu.edu.
Sean X SunJohns Hopkins Physical Sciences-Oncology Center, Johns Hopkins University, Baltimore, MD 21218, USA Department of Mechanical Engineering, Johns Hopkins University, Baltimore, MD 21218, USA wirtz@jhu.edu ssun@jhu.edu.

Funding

TRANS NETWORK PROJECTSU54CA143868 · NCI · JOHNS HOPKINS UNIVERSITY · PI WIRTZ, DENIS · 2009 to 2013
$13.7M
NCI NIH HHS 1U54CA143868NCI NIH HHS U54 CA143868
6 · The paper itself

Abstract

Alterations in nuclear morphology are closely associated with essential cell functions, such as cell motility and polarization, and correlate with a wide range of human diseases, including cancer, muscular dystrophy, dilated cardiomyopathy and progeria. However, the mechanics and forces that shape the nucleus are not well understood. Here, we demonstrate that when an adherent cell is detached from its substratum, the nucleus undergoes a large volumetric reduction accompanied by a morphological transition from an almost smooth to a heavily folded surface. We develop a mathematical model that systematically analyzes the evolution of nuclear shape and volume. The analysis suggests that the pressure difference across the nuclear envelope, which is influenced by changes in cell volume and regulated by microtubules and actin filaments, is a major factor determining nuclear morphology. Our results show that physical and chemical properties of the extracellular microenvironment directly influence nuclear morphology and suggest that there is a direct link between the environment and gene regulation.

Indexed as

Cell NucleusCell Nucleus ShapeCell Nucleus SizeCellular MicroenvironmentModels, TheoreticalActinsAnimalsCell LineFibroblastsHumansMiceMicrotubulesActinsCell nucleusMechanicsMulti-bifurcationsVolume change

Identifiers

PMID26243474
PMCPMC4582398

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.