ArticleMalaria journal2015
Improvement of culture conditions for long-term in vitro culture of Plasmodium vivax.
Article in Malaria journal, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed.
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- Complement receptor 1 is the human erythrocyte receptor forProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Development of a Plasmodium vivax biobank for functional ex vivo assays.Malaria journal · 2023Article
- Biology and epidemiology ofParasite epidemiology and control · 2023Review
- The direct binding ofProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Editorial: Reviews in neglected tropical infectious diseases.Frontiers in microbiology · 2023Article
- Culture and transfection: Two major bottlenecks in understandingFrontiers in microbiology · 2023Review
- The challenges ofFrontiers in immunology · 2022Article
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- Generation of red blood cells from stem cells: Achievements, opportunities and perspectives for malaria research.Frontiers in cellular and infection microbiology · 2022Review
- Infectivity of Symptomatic Malaria Patients toFrontiers in cellular and infection microbiology · 2021Article
- Home Sweet Home:Frontiers in cellular and infection microbiology · 2021Review
- Identification of the RecombinantFrontiers in cell and developmental biology · 2021Article
- Article
- On the survival of 48 h Plasmodium vivax Aotus monkey-derived ex vivo cultures: the role of leucocytes filtration and chemically defined lipid concentrate media supplementation.Malaria journal · 2020Article
- Genetic diversity and neutral selection in Plasmodium vivax erythrocyte binding protein correlates with patient antigenicity.PLoS neglected tropical diseases · 2020Article
- Plasmodium vivax liver stage assay platforms using Indian clinical isolates.Malaria journal · 2020Article
- Plasma-derived extracellular vesicles from Plasmodium vivax patients signal spleen fibroblasts via NF-kB facilitating parasite cytoadherence.Nature communications · 2020Article
- A Way Forward for Culturing Plasmodium vivax.Trends in parasitology · 2020Review
- From marginal to essential: the golden thread between nutrient sensing, medium composition and Plasmodium vivax maturation in in vitro culture.Malaria journal · 2019Review
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8 authors.
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Abstract
backgroundThe study of the biology, transmission and pathogenesis of Plasmodium vivax is hindered due to the lack of a robustly propagating, continuous culture of this parasite. The current culture system for P. vivax parasites still suffered from consistency and difficulties in long-term maintenance of parasites in culture and for providing sufficient biological materials for studying parasite biology. Therefore, further improvement of culture conditions for P. vivax is needed.
methodsClinical samples were collected from patients diagnosed with P. vivax in western Thailand. Leukocyte-depleted P. vivax infected blood samples were cultured in a modified McCoy's 5A medium at 5% haematocrit under hypoxic condition (5% O2, 5% CO2, and 90% N2). Reticulocytes purified from adult peripheral blood were added daily to maintain 4% reticulocytes. Parasites were detected by microscopic examination of Giemsa-stained smears and molecular methods.
resultsThe effects of culture variables were first analysed in order to improve the culture conditions for P. vivax. Through analysis of the sources of host reticulocytes and nutrients of culture medium, the culture conditions better supporting in vitro growth and maturation of the parasites were identified. Using this system, three of 30 isolates could be maintained in vitro for over 26 months albeit parasite density is low.
conclusionsBased on the analysis of different culture variables, an improved and feasible protocol for continuous culture of P. vivax was developed.
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