ArticleNano letters2015
A Protein Rotaxane Controls the Translocation of Proteins Across a ClyA Nanopore.
Article in Nano letters, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed.
- Translocation of linearized full-length proteins through an engineered nanopore under opposing electrophoretic force.Nature biotechnology · 2024Article
- Enzyme-less nanopore detection of post-translational modifications within long polypeptides.Nature nanotechnology · 2023Article
- Nanopore sensors for single molecular protein detection: Research progress based on computer simulations.IET nanobiotechnology · 2023Review
- Single-molecule fingerprinting of protein-drug interaction using a funneled biological nanopore.Nature communications · 2023Article
- PlyAB Nanopores Detect Single Amino Acid Differences in Folded Haemoglobin from Blood.Angewandte Chemie (International ed. in English) · 2022Article
- β-Barrel Nanopores with an Acidic-Aromatic Sensing Region Identify Proteinogenic Peptides at Low pH.ACS nano · 2022Article
- Single-Molecule Sampling of Dihydrofolate Reductase Shows Kinetic Pauses and an Endosteric Effect Linked to Catalysis.ACS catalysis · 2022Article
- Nanopore Technology for the Application of Protein Detection.Nanomaterials (Basel, Switzerland) · 2021Review
- Autonomous and Active Transport Operated by an Entropic DNA Piston.Nano letters · 2021Article
- Biological Nanopores: Engineering on Demand.Life (Basel, Switzerland) · 2021Review
- Directional conformer exchange in dihydrofolate reductase revealed by single-molecule nanopore recordings.Nature chemistry · 2020Article
- Article
- Resolving Chemical Modifications to a Single Amino Acid within a Peptide Using a Biological Nanopore.ACS nano · 2019Article
- Engineering and Modeling the Electrophoretic Trapping of a Single Protein Inside a Nanopore.ACS nano · 2019Article
- Article
- FraC nanopores with adjustable diameter identify the mass of opposite-charge peptides with 44 dalton resolution.Nature communications · 2019Article
- Comparative biosensing of glycosaminoglycan hyaluronic acid oligo- and polysaccharides using aerolysin andThe European physical journal. E, Soft matter · 2018Article
- The Utility of Nanopore Technology for Protein and Peptide Sensing.Proteomics · 2018Review
- Identification of single amino acid differences in uniformly charged homopolymeric peptides with aerolysin nanopore.Nature communications · 2018Article
- Real-Time Conformational Changes and Controlled Orientation of Native Proteins Inside a Protein Nanoreactor.Journal of the American Chemical Society · 2017Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Rotaxanes, pseudorotaxanes, and catenanes are supramolecular complexes with potential use in nanomachinery, molecular computing, and single-molecule studies. Here we constructed a protein rotaxane in which a polypeptide thread is encircled by a Cytolysin A (ClyA) nanopore and capped by two protein stoppers. The rotaxane could be switched between two states. At low negative applied potentials (<-50 mV) one of the protein stoppers resided inside the nanopore indefinitely. Under this configuration the rotaxane prevents the diffusion of protein molecules across the lipid bilayer and provides a useful platform for single-molecule analysis. High negative applied potentials (-100 mV) dismantled the interlocked rotaxane system by the forceful translocation of the protein stopper, allowing new proteins to be trapped inside or transported across the nanopore. The observed voltage threshold for the translocation of the protein stopper through the nanopore related well to the biphasic voltage dependence of the residence time measured for the freely diffusing protein stopper. We propose a model in which molecules translocate through a nanopore when the average dwell time decreases with the applied potential.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.