ArticlePlant cell reports2015
Sp-miR396a-5p acts as a stress-responsive genes regulator by conferring tolerance to abiotic stresses and susceptibility to Phytophthora nicotianae infection in transgenic tobacco.
Article in Plant cell reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
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Who cites it
64 citing papers in PubMed, 159 citations in OpenAlex.
- The multifaceted regulatory network of microRNA396: Orchestrating plant plasticity through coordinating development and various stresses.Journal of integrative plant biology · 2026Review
- The E3 ubiquitin ligase RHB1A and the transcription factor GRF1 form a regulatory module that orchestrates the trade-off between photosynthetic growth and salt tolerance in poplar.Plant communications · 2026Article
- Targeted Regulation ofPlants (Basel, Switzerland) · 2025Article
- Genome-wide identification and characterization of DUF789 genes in cotton: implications for fibre development.BMC plant biology · 2025Article
- Non-coding RNAs in plant stress responses: molecular insights and agricultural applications.Plant biotechnology journal · 2025Review
- Identification and Network Construction of mRNAs, miRNAs, lncRNAs, and circRNAs in Sweetpotato (International journal of molecular sciences · 2025Article
- Article
- Genome‑wide identifcation and expression analysis of growth-regulating factors under drought in Brassica juncea.Scientific reports · 2024Article
- Blocking miR396 activity by overexpression MIM396 improved switchgrass tiller number and biomass yield.Biotechnology for biofuels and bioproducts · 2024Article
- Creating Climate-Resilient Crops by Increasing Drought, Heat, and Salt Tolerance.Plants (Basel, Switzerland) · 2024Review
- Article
- microRNAs: Key Regulators in Plant Responses to Abiotic and Biotic Stresses via Endogenous and Cross-Kingdom Mechanisms.International journal of molecular sciences · 2024Review
- Genome-wide identification of the GRF family in sweet orange (Citrus sinensis) and functional analysis of the CsGRF04 in response to multiple abiotic stresses.BMC genomics · 2024Article
- BpGRP1 acts downstream of BpmiR396c/BpGRF3 to confer salt tolerance in Betula platyphylla.Plant biotechnology journal · 2024Article
- EnhancingFrontiers in plant science · 2024Article
- Drought-Induced miRNA Expression Correlated with Heavy Metal, Phenolic Acid, and Protein and Nitrogen Levels in Five Chickpea Genotypes.ACS omega · 2023Article
- MicroRNA2871b of Dongxiang Wild Rice (International journal of molecular sciences · 2023Article
- Endoplasmic reticulum stress-responsive microRNAs are involved in the regulation of abiotic stresses in wheat.Plant cell reports · 2023Article
- Small RNAs: Efficient and miraculous effectors that play key roles in plant-microbe interactions.Molecular plant pathology · 2023Review
- Article
4 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
key messageOverexpression of Sp-miR396a-5p in tobacco increased tolerance to salt, drought, cold stress and susceptibility to Phytophthora nicotianae infection. MicroRNA396 (miR396) is one of the conserved microRNA families in plants, and it targeted growth-regulating factors (GRFs) family. The GRF transcription factors are associated with growth and stress responses. However, the molecular mechanisms of miR396 responding to environmental stresses are elusive. The purpose of this study was to explore the function of tomato miR396a-5p (Sp-miR396a-5p) in Solanaceae responses to abiotic and biotic stresses. We showed that Sp-miR396a-5p transcript levels were up-regulated under salt and drought stresses and down-regulated after Phytophthora infestans (P. infestans) infection. Consistently, overexpression of Sp-miR396a-5p in tobacco enhanced its tolerance to salt, drought and cold stresses. Additionally, the expression of Sp-miR396a-5p was found to be down-regulated under pathogen-related biotic stress. Tobacco plants overexpressing Sp-miR396a-5p showed increased susceptibility to Phytophthora nicotianae (P. nicotianae) infection. Physiological analysis indicated that Sp-miR396a-5p overexpression enhanced osmoregulation and decreased production of reactive oxygen species (ROS). Furthermore, four Sp-miR396a-5p target genes, NtGRF1, NtGRF3, NtGRF7 and NtGRF8, were down-regulated in these plants. Our results suggested that Sp-miR396a-5p plays critical roles in both abiotic stresses through targeting NtGRF7-regulated expression of osmotic stress-responsive genes and pathogen infection via the regulatory networks of NtGRF1 and NtGRF3.
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