ArticleNucleic acids research2015
A novel method for the multiplexed target enrichment of MinION next generation sequencing libraries using PCR-generated baits.
Article in Nucleic acids research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Evaluation of a Probe-Based Enrichment Protocol for Nanopore Sequencing of Zoonotic Viruses.Viruses · 2025Article
- Hybridization capture long-read sequencing and de novo assembly of homologous haplotypes: a comprehensive hemophilia test.Blood advances · 2025Article
- Real-time and programmable transcriptome sequencing with PROFIT-seq.Nature cell biology · 2024Article
- An integrated strategy involving high-throughput sequencing to characterize an unknown GM wheat event in Canada.Plant biotechnology journal · 2024Article
- Assessing the efficacy of target adaptive sampling long-read sequencing through hereditary cancer patient genomes.NPJ genomic medicine · 2024Article
- Beyond assembly: the increasing flexibility of single-molecule sequencing technology.Nature reviews. Genetics · 2023Review
- Long-Read Sequencing in Blood Group Genetics.Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie · 2023Review
- High-throughput method for the hybridisation-based targeted enrichment of long genomic fragments for PacBio third-generation sequencing.NAR genomics and bioinformatics · 2022Article
- Article
- Targeted long-read sequencing identifies missing disease-causing variation.American journal of human genetics · 2021Article
- Assessment of Viral Targeted Sequence Capture Using Nanopore Sequencing Directly from Clinical Samples.Viruses · 2020Article
- Next-generation sequencing of 35 RHD variants in 16 253 serologically D- pregnant women in the Finnish population.Blood advances · 2020Article
- ORF Capture-Seq as a versatile method for targeted identification of full-length isoforms.Nature communications · 2020Article
- Review
- Next-Generation Sequencing Technologies in Blood Group Typing.Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie · 2020Review
- Shotgun metagenome data of a defined mock community using Oxford Nanopore, PacBio and Illumina technologies.Scientific data · 2019Article
- Real-Time Selective Sequencing with RUBRIC: Read Until with Basecall and Reference-Informed Criteria.Scientific reports · 2019Article
- Nanopore sequencing and full genome de novo assembly of human cytomegalovirus TB40/E reveals clonal diversity and structural variations.BMC genomics · 2018Article
- Multiplexed Nanopore Sequencing of HLA-B Locus in Māori and Pacific Island Samples.Frontiers in genetics · 2018Article
- Impact of Interferon-α Receptor-1 Promoter Polymorphisms on the Transcriptome of the Hepatitis B Virus-Associated Hepatocellular Carcinoma.Frontiers in immunology · 2018Article
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2 authors.
Funding
Abstract
The enrichment of targeted regions within complex next generation sequencing libraries commonly uses biotinylated baits to capture the desired sequences. This method results in high read coverage over the targets and their flanking regions. Oxford Nanopore Technologies recently released an USB3.0-interfaced sequencer, the MinION. To date no particular method for enriching MinION libraries has been standardized. Here, using biotinylated PCR-generated baits in a novel approach, we describe a simple and efficient way for multiplexed enrichment of MinION libraries, overcoming technical limitations related with the chemistry of the sequencing-adapters and the length of the DNA fragments. Using Phage Lambda and Escherichia coli as models we selectively enrich for specific targets, significantly increasing the corresponding read-coverage, eliminating unwanted regions. We show that by capturing genomic fragments, which contain the target sequences, we recover reads extending targeted regions and thus can be used for the determination of potentially unknown flanking sequences. By pooling enriched libraries derived from two distinct E. coli strains and analyzing them in parallel, we demonstrate the efficiency of this method in multiplexed format. Crucially we evaluated the optimal bait size for large fragment libraries and we describe for the first time a standardized method for target enrichment in MinION platform.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.