ArticleProceedings of the National Academy of Sciences of the United States of America2015
A globally distributed mobile genetic element inhibits natural transformation of Vibrio cholerae.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
35 citing papers in PubMed, 63 citations in OpenAlex.
- Genomic and functional dissection of natural transformation-related genes inMicrobiology spectrum · 2026Article
- Article
- Naturally competent bacteria and their genetic parasites-a battle for control over horizontal gene transfer?FEMS microbiology reviews · 2025Review
- Intragenomic conflicts with plasmids and chromosomal mobile genetic elements drive the evolution of natural transformation within species.PLoS biology · 2024Article
- Manipulation of natural transformation by AbaR-type islands promotes fixation of antibiotic resistance inProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Emergence of multidrug resistant, ctx negative seventh pandemic Vibrio cholerae O1 El Tor sequence type (ST) 69 in coastal water of Kerala, India.Scientific reports · 2024Article
- Role of Bacteriophages in the Evolution of Pathogenic Vibrios and Lessons for Phage Therapy.Advances in experimental medicine and biology · 2023Article
- Genomes of Vibrio metoecus co-isolated with Vibrio cholerae extend our understanding of differences between these closely related species.Gut pathogens · 2022Article
- Evolutionary Pathways and Trajectories in Antibiotic Resistance.Clinical microbiology reviews · 2021Article
- A Strategy for the Rapid Development of a SafeInternational journal of molecular sciences · 2021Article
- Temporal shifts in antibiotic resistance elements govern phage-pathogen conflicts.Science (New York, N.Y.) · 2021Article
- Natural Transformation ofFrontiers in microbiology · 2021Article
- Comparison of chitin-induced natural transformation in pandemic Vibrio cholerae O1 El Tor strains.Environmental microbiology · 2020Article
- The Large pBS32/pLS32 Plasmid of Ancestral Bacillus subtilis.Journal of bacteriology · 2020Review
- Article
- Diverse conjugative elements silence natural transformation inProceedings of the National Academy of Sciences of the United States of America · 2019Article
- Neighbor predation linked to natural competence fosters the transfer of large genomic regions ineLife · 2019Article
- Conversion of aFrontiers in microbiology · 2019Article
- Vibrio cholerae motility exerts drag force to impede attack by the bacterial predator Bdellovibrio bacteriovorus.Nature communications · 2018Article
- ComM is a hexameric helicase that promotes branch migration during natural transformation in diverse Gram-negative species.Nucleic acids research · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 4 institutions in 1 country.
Funding
Abstract
Natural transformation is one mechanism of horizontal gene transfer (HGT) in Vibrio cholerae, the causative agent of cholera. Recently, it was found that V. cholerae isolates from the Haiti outbreak were poorly transformed by this mechanism. Here, we show that an integrating conjugative element (ICE)-encoded DNase, which we name IdeA, is necessary and sufficient for inhibiting natural transformation of Haiti outbreak strains. We demonstrate that IdeA inhibits this mechanism of HGT in cis via DNA endonuclease activity that is localized to the periplasm. Furthermore, we show that natural transformation between cholera strains in a relevant environmental context is inhibited by IdeA. The ICE encoding IdeA is globally distributed. Therefore, we analyzed the prevalence and role for this ICE in limiting natural transformation of isolates from Bangladesh collected between 2001 and 2011. We found that IdeA(+) ICEs were nearly ubiquitous in isolates from 2001 to 2005; however, their prevalence decreased to ∼40% from 2006 to 2011. Thus, IdeA(+) ICEs may have limited the role of natural transformation in V. cholerae. However, the rise in prevalence of strains lacking IdeA may now increase the role of this conserved mechanism of HGT in the evolution of this pathogen.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.