ArticleProceedings of the National Academy of Sciences of the United States of America2015
At14a-Like1 participates in membrane-associated mechanisms promoting growth during drought in Arabidopsis thaliana.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 50 citations in OpenAlex.
- Characterization, evolution, and expression profiling of cotton DUF677 protein family and their potential role in drought and salt stress response.BMC plant biology · 2026Article
- Crops exposed to extreme conditions: perspectives of gene editing to improve stress tolerance.Plant cell reports · 2026Review
- Dual and spatially resolved drought responses in the Arabidopsis leaf mesophyll revealed by single-cell transcriptomics.The New phytologist · 2025Article
- Proteomics revealed novel functions and drought tolerance of Arabidopsis thaliana protein kinase ATG1.BMC biology · 2025Article
- Transcriptional repression ofPlant direct · 2024Article
- Integrated Transcriptome and Proteome Analysis Reveals the Regulatory Mechanism of Root Growth by Protein Disulfide Isomerase in Arabidopsis.International journal of molecular sciences · 2024Article
- Upland rice genomic signatures of adaptation to drought resistance and navigation to molecular design breeding.Plant biotechnology journal · 2024Article
- Article
- 2 in 1 Vectors Improve in Planta BiFC and FRET Analysis.Methods in molecular biology (Clifton, N.J.) · 2024Article
- The Arabidopsis thaliana integrin-like gene AT14A improves drought tolerance in Solanum lycopersicum.Journal of plant research · 2023Article
- An intrinsically disordered region-containing protein mitigates the drought-growth trade-off to boost yields.Plant physiology · 2023Article
- Specialized endoplasmic reticulum-derived vesicles in plants: Functional diversity, evolution, and biotechnological exploitation.Journal of integrative plant biology · 2022Review
- The Cellular and Subcellular Organization of the Glucosinolate-Myrosinase System against Herbivores and Pathogens.International journal of molecular sciences · 2022Review
- Expression Characterization ofInternational journal of molecular sciences · 2022Article
- Altering the balance between AOX1A and NDB2 expression affects a common set of transcripts in Arabidopsis.Frontiers in plant science · 2022Article
- Article
- The Beginning of the End: Initial Steps in the Degradation of Plasma Membrane Proteins.Frontiers in plant science · 2020Review
- Highly ABA-Induced 1 (HAI1)-Interacting protein HIN1 and drought acclimation-enhanced splicing efficiency at intron retention sites.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- A Family of NAI2-Interacting Proteins in the Biogenesis of the ER Body and Related Structures.Plant physiology · 2019Article
- Low Water Potential and At14a-Like1 (AFL1) Effects on Endocytosis and Actin Filament Organization.Plant physiology · 2019Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Limited knowledge of how plants regulate their growth and metabolism in response to drought and reduced soil water potential has impeded efforts to improve stress tolerance. Increased expression of the membrane-associated protein At14a-like1 (AFL1) led to increased growth and accumulation of the osmoprotective solute proline without negative effects on unstressed plants. Conversely, inducible RNA-interference suppression of AFL1 decreased growth and proline accumulation during low water potential while having no effect on unstressed plants. AFL1 overexpression lines had reduced expression of many stress-responsive genes, suggesting AFL1 may promote growth in part by suppression of negative regulatory genes. AFL1 interacted with the endomembrane proteins protein disulfide isomerase 5 (PDI5) and NAI2, with the PDI5 interaction being particularly increased by stress. PDI5 and NAI2 are negative regulatory factors, as pdi5, nai2, and pdi5-2nai2-3 mutants had increased growth and proline accumulation at low water potential. AFL1 also interacted with Adaptor protein2-2A (AP2-2A), which is part of a complex that recruits cargo proteins and promotes assembly of clathrin-coated vesicles. AFL1 colocalization with clathrin light chain along the plasma membrane, together with predictions of AFL1 structure, were consistent with a role in vesicle formation or trafficking. Fractionation experiments indicated that AFL1 is a peripheral membrane protein associated with both plasma membrane and endomembranes. These data identify classes of proteins (AFL1, PDI5, and NAI2) not previously known to be involved in drought signaling. AFL1-predicted structure, protein interactions, and localization all indicate its involvement in previously uncharacterized membrane-associated drought sensing or signaling mechanisms.
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