Evidence map›Paper›PMID 26240167›Full record

ArticleGlycobiology2015

Analysis of site-specific N-glycan remodeling in the endoplasmic reticulum and the Golgi.

Ivan Hang, Chia-wei Lin, Oliver C Grant, Susanna Fleurkens, Thomas K Villiger, Miroslav Soos, Massimo Morbidelli, Robert J Woods, Robert Gauss, Markus Aebi

Open access · bronzeAbstract read
In one paragraph

Article in Glycobiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.

0numbers the graph read from it
0cells of the map it votes in
31citing papers in PubMed
3.5field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

31 citing papers in PubMed, 70 citations in OpenAlex.

  1. High-Throughput Proteomic and Glycoproteomic Analyses in Benign Prostatic Hyperplasia.Journal of the American Society for Mass Spectrometry · 2026
    Article
  2. Functions ofJournal of fungi (Basel, Switzerland) · 2025
    Review
  3. Article
  4. Review
  5. Review
  6. Review
  7. Article
  8. Principles of SARS-CoV-2 glycosylation.Current opinion in structural biology · 2022
    Review
  9. Article
  10. Review
  11. Article
  12. Article
  13. Article
  14. Engineering of Yeast Glycoprotein Expression.Advances in biochemical engineering/biotechnology · 2021
    Article
  15. Article
  16. Visualization of the HIV-1 Env glycan shield across scales.Proceedings of the National Academy of Sciences of the United States of America · 2020
    Article
  17. Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 3 countries.

Ivan HangInstitute of Microbiology, Department of Biology.
Chia-wei LinInstitute of Microbiology, Department of Biology.
Oliver C GrantComplex Carbohydrate Research Center, University of Georgia, Athens, GA 30602, USA.
Susanna FleurkensInstitute of Microbiology, Department of Biology.
Thomas K VilligerInstitute for Chemical and Bioengineering, Department of Chemistry and Applied Biosciences, Swiss Federal Institute of Technology, ETH Zurich, CH-8093 Zurich, Switzerland.
Miroslav SoosInstitute for Chemical and Bioengineering, Department of Chemistry and Applied Biosciences, Swiss Federal Institute of Technology, ETH Zurich, CH-8093 Zurich, Switzerland.
Massimo MorbidelliInstitute for Chemical and Bioengineering, Department of Chemistry and Applied Biosciences, Swiss Federal Institute of Technology, ETH Zurich, CH-8093 Zurich, Switzerland.
Robert J WoodsComplex Carbohydrate Research Center, University of Georgia, Athens, GA 30602, USA.
Robert GaussInstitute of Microbiology, Department of Biology markus.aebi@micro.biol.ethz.ch robert.gauss@micro.biol.ethz.ch.
Markus AebiInstitute of Microbiology, Department of Biology markus.aebi@micro.biol.ethz.ch robert.gauss@micro.biol.ethz.ch.
Institute of Microbiology · CNETH Zurich · CHUniversity of Georgia · US

Funding

TR&D3. Approaches to Proteoglycan Function at Biochemical and Cellular LevelsP41GM103390 · NIGMS · UNIVERSITY OF GEORGIA · PI MOREMEN, KELLEY W. · 2012 to 2019
$14.5M
Integrating Experiment and Theory to Characterize Diagnostic Antibody SpecificityR01GM094919 · NIGMS · UNIVERSITY OF GEORGIA · PI WOODS, ROBERT J · 2010 to 2013
$1.7M
NIGMS NIH HHS P41 GM103390NIGMS NIH HHS P41GM103390NIGMS NIH HHS R01 GM094919NIGMS NIH HHS R01GM094919
6 · The paper itself

Abstract

The hallmark of N-linked protein glycosylation is the generation of diverse glycan structures in the secretory pathway. Dynamic, non-template-driven processes of N-glycan remodeling in the endoplasmic reticulum and the Golgi provide the cellular setting for structural diversity. We applied newly developed mass spectrometry-based analytics to quantify site-specific N-glycan remodeling of the model protein Pdi1p expressed in insect cells. Molecular dynamics simulation, mutational analysis, kinetic studies of in vitro processing events and glycan flux analysis supported the defining role of the protein in N-glycan processing.

Indexed as

Protein Processing, Post-TranslationalAnimalsEndoplasmic ReticulumGlycosylationGolgi ApparatusPolysaccharidesProtein Disulfide-IsomerasesSaccharomyces cerevisiae ProteinsSf9 CellsSpodopteraPDI1 protein, S cerevisiaePolysaccharidesProtein Disulfide-IsomerasesSaccharomyces cerevisiae Proteinsendoplasmic reticulum glycosylationGolgiGolgi glycosylationsite-specific glycosylation

Identifiers

PMID26240167
PMCPMC4634314
OpenAlexW2218010822

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.