ArticleScientific reports2015
A highly sensitive and specific method for the screening detection of genetically modified organisms based on digital PCR without pretreatment.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Development and Validation of a New Robust Detection Method for Low-Content DNA Using ΔΔCq-Based Real-Time PCR with Optimized Standard Plasmids as a Control Sample.Analytical chemistry · 2022Article
- Digital PCR: What Relevance to Plant Studies?Biology · 2020Review
- Multiplex quantitative PCR for single-reaction genetically modified (GM) plant detection and identification of false-positive GM plants linked to Cauliflower mosaic virus (CaMV) infection.BMC biotechnology · 2019Article
- A high-throughput and ultrasensitive identification methodology for unauthorized GMP component based on suspension array and logical calculator.Scientific reports · 2019Article
- Critical assessment of digital PCR for the detection and quantification of genetically modified organisms.Analytical and bioanalytical chemistry · 2018Review
- Improved Detection and Monitoring of Fungicide Resistance inFrontiers in microbiology · 2018Article
- Colorimetric detection of genetically modified organisms based on exonuclease III-assisted target recycling and hemin/G-quadruplex DNAzyme amplification.Mikrochimica acta · 2017Article
- Review
- Evaluation of the performance of quantitative detection of the Listeria monocytogenes prfA locus with droplet digital PCR.Analytical and bioanalytical chemistry · 2016Article
- A Novel Pretreatment-Free Duplex Chamber Digital PCR Detection System for the Absolute Quantitation of GMO Samples.International journal of molecular sciences · 2016Article
- Article
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Authors and funding
10 authors.
Funding
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Abstract
Digital PCR has developed rapidly since it was first reported in the 1990 s. It was recently reported that an improved method facilitated the detection of genetically modified organisms (GMOs). However, to use this improved method, the samples must be pretreated, which could introduce inaccuracy into the results. In our study, we explored a pretreatment-free digital PCR detection method for the screening for GMOs. We chose the CaMV35s promoter and the NOS terminator as the templates in our assay. To determine the specificity of our method, 9 events of GMOs were collected, including MON810, MON863, TC1507, MIR604, MIR162, GA21, T25, NK603 and Bt176. Moreover, the sensitivity, intra-laboratory and inter-laboratory reproducibility of our detection method were assessed. The results showed that the limit of detection of our method was 0.1%, which was lower than the labeling threshold level of the EU. The specificity and stability among the 9 events were consistent, respectively. The intra-laboratory and inter-laboratory reproducibility were both good. Finally, the perfect fitness for the detection of eight double-blind samples indicated the good practicability of our method. In conclusion, the method in our study would allow more sensitive, specific and stable screening detection of the GMO content of international trading products.
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