ArticleInfection and immunity2015
Fibronectin Binding Proteins SpsD and SpsL Both Support Invasion of Canine Epithelial Cells by Staphylococcus pseudintermedius.
Article in Infection and immunity, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Emerging threats and zoonotic implications of antibiotic-resistant Staphylococcus spp. in domesticated cats: a review.Antonie van Leeuwenhoek · 2026Review
- Bacteriophage and Fusidic Acid Have Synergistic Effect Against Meticillin-Resistant Staphylococcus pseudintermedius in Ex Vivo Canine Dermis Model.Veterinary dermatology · 2026Article
- IntratumoralAnimals : an open access journal from MDPI · 2026Article
- Staphylococcal internalization into osteoblasts: a partially conserved mechanism across the genus.mBio · 2026Article
- The dynamic interplay betweenFrontiers in cellular and infection microbiology · 2026Review
- Staphylococcus pseudintermedius induces pyroptosis of canine corneal epithelial cells by activating the ROS-NLRP3 signalling pathway.Virulence · 2024Article
- Staphylococcus aureus can use an alternative pathway to be internalized by osteoblasts in absence of β1 integrins.Scientific reports · 2024Article
- Investigation on biofilm composition and virulence traits of S. pseudintermedius isolated from infected and colonized dogs.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024Article
- Engagement of αFrontiers in microbiology · 2024Article
- Bacteriocins Revitalize Non-Effective Penicillin G to Overcome Methicillin-ResistantAntibiotics (Basel, Switzerland) · 2022Article
- Prevalence ofMicroorganisms · 2022Article
- Methicillin-resistantVirulence · 2021Article
- Article
- Virulence factors in coagulase-positive staphylococci of veterinary interest other thanThe veterinary quarterly · 2020Review
- Staphylococcal Protein A (mSphere · 2020Article
- Force-clamp spectroscopy identifies a catch bond mechanism in a Gram-positive pathogen.Nature communications · 2020Article
- Article
- Identification and Characterization of Staphylococcus delphini Internalization Pathway in Nonprofessional Phagocytic Cells.Infection and immunity · 2020Article
- The Multivalent Role of Fibronectin-Binding Proteins A and B (FnBPA and FnBPB) ofFrontiers in microbiology · 2020Review
- Staphylococcus pseudintermedius: an undocumented, emerging pathogen in humans.GMS hygiene and infection control · 2020Review
Corrections and comments
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Authors and funding
11 authors.
Funding
Abstract
In this study, we investigated the cell wall-anchored fibronectin-binding proteins SpsD and SpsL from the canine commensal and pathogen Staphylococcus pseudintermedius for their role in promoting bacterial invasion of canine progenitor epidermal keratinocytes (CPEK). Invasion was examined by the gentamicin protection assay and fluorescence microscopy. An ΔspsD ΔspsL mutant of strain ED99 had a dramatically reduced capacity to invade CPEK monolayers, while no difference in the invasion level was observed with single mutants. Lactococcus lactis transformed with plasmids expressing SpsD and SpsL promoted invasion, showing that both proteins are important. Soluble fibronectin was required for invasion, and an RGD-containing peptide or antibodies recognizing the integrin α5β1 markedly reduced invasion, suggesting an important role for the integrin in this process. Src kinase inhibitors effectively blocked internalization, suggesting a functional role for the kinase in invasion. In order to identify the minimal fibronectin-binding region of SpsD and SpsL involved in the internalization process, recombinant fragments of both proteins were produced. The SpsD520-846 and SpsL538-823 regions harboring the major fibronectin-binding sites inhibited S. pseudintermedius internalization. Finally, the effects of staphylococcal invasion on the integrity of different cell lines were examined. Because SpsD and SpsL are critical factors for adhesion and invasion, blocking these processes could provide a strategy for future approaches to treating infections.
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