ArticleBMC genomics2015
High-throughput and quantitative genome-wide messenger RNA sequencing for molecular phenotyping.
Article in BMC genomics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed.
- Affordable, accurate and unbiased RNA sequencing by manual library miniaturization: A case study in barley.Plant biotechnology journal · 2023Article
- Loss of slc39a14 causes simultaneous manganese hypersensitivity and deficiency in zebrafish.Disease models & mechanisms · 2022Article
- Allele-specific gene expression can underlie altered transcript abundance in zebrafish mutants.eLife · 2022Article
- Effects of Β‒Blocker Administration on Cardiac Function: A Coronary Computed Tomography Angiography Study.Current medical imaging · 2022Article
- Integrated analysis highlights multiple long non‑coding RNAs and their potential roles in the progression of human esophageal squamous cell carcinoma.Oncology reports · 2019Article
- The gene regulatory basis of genetic compensation during neural crest induction.PLoS genetics · 2019Article
- Fishing forward and reverse: Advances in zebrafish phenomics.Mechanisms of development · 2018Review
- Elimination of PCR duplicates in RNA-seq and small RNA-seq using unique molecular identifiers.BMC genomics · 2018Article
- Article
- UMI-tools: modeling sequencing errors in Unique Molecular Identifiers to improve quantification accuracy.Genome research · 2017Article
- Global open data management in metabolomics.Current opinion in chemical biology · 2017Review
- DANIO-CODE: Toward an Encyclopedia of DNA Elements in Zebrafish.Zebrafish · 2016Article
- The Ribosome Biogenesis Protein Nol9 Is Essential for Definitive Hematopoiesis and Pancreas Morphogenesis in Zebrafish.PLoS genetics · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
15 authors.
Funding
Abstract
backgroundWe present a genome-wide messenger RNA (mRNA) sequencing technique that converts small amounts of RNA from many samples into molecular phenotypes. It encompasses all steps from sample preparation to sequence analysis and is applicable to baseline profiling or perturbation measurements.
resultsMultiplex sequencing of transcript 3' ends identifies differential transcript abundance independent of gene annotation. We show that increasing biological replicate number while maintaining the total amount of sequencing identifies more differentially abundant transcripts.
conclusionsThis method can be implemented on polyadenylated RNA from any organism with an annotated reference genome and in any laboratory with access to Illumina sequencing.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.