Evidence map›Paper›PMID 26238050›Full record

ReviewAdvances in experimental medicine and biology2015

Combining Membrane Potential Imaging with Other Optical Techniques.

Nadia Jaafari, Kaspar E Vogt, Peter Saggau, Loew M Leslie, Dejan Zecevic, Marco Canepari

Abstract readReview
In one paragraph

Review in Advances in experimental medicine and biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Isomerically Pure Tetramethylrhodamine Voltage Reporters.Journal of the American Chemical Society · 2016
    Article
  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Nadia JaafariInserm U836, Grenoble Institute of Neuroscience, Team 3, Grenoble Cedex 09, France.
Kaspar E Vogt
Peter Saggau
Loew M Leslie
Dejan Zecevic
Marco Canepari

Funding

Electrical role of dendritic spinesR01MH106906 · NIMH · YALE UNIVERSITY · PI ZECEVIC, DEJAN P · 2015 to 2019
$2.0M
NIMH NIH HHS R01 MH106906
6 · The paper itself

Abstract

Membrane potential imaging using voltage-sensitive dyes can be combined with other optical techniques for a variety of applications. Combining voltage imaging with Ca2+ imaging allows correlating membrane potential changes with intracellular Ca2+ signals or with Ca2+ currents. Combining voltage imaging with uncaging techniques allows analyzing electrical signals elicited by photorelease of a particular molecule. This approach is also a useful tool to calibrate the change in fluorescence intensity in terms of membrane potential changes from different sites permitting spatial mapping of electrical activity. Finally, combining voltage imaging with optogenetics, in particular with channelrhodopsin stimulation, opens the gate to novel investigations of brain circuitries by allowing measurements of synaptic signals mediated by specific sets of neurons. Here we describe in detail the methods of membrane potential imaging in combination with other optical techniques and discus some important applications.

Indexed as

AnimalsCalciumCalcium SignalingChannelrhodopsinsFluorescent DyesGlutamic AcidMembrane PotentialsMiceNerve NetNeuronsOptical ImagingOptogeneticsSingle-Cell AnalysisSynapsesVoltage-Sensitive Dye ImagingCalciumChannelrhodopsinsFluorescent DyesGlutamic Acid

Identifiers

PMID26238050
PMCPMC5675139

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.