Evidence map›Paper›PMID 26237675›Full record

ArticleCurrent protocols in protein science2015

Applications of Lipid Nanodiscs for the Study of Membrane Proteins by Surface Plasmon Resonance.

Meg Trahey, Mavis Jiarong Li, Hyewon Kwon, Erica L Woodahl, Wynton D McClary, William M Atkins

Open access · greenAbstract read
In one paragraph

Article in Current protocols in protein science, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
0.9field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 27 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Nanodiscs: A toolkit for membrane protein science.Protein science : a publication of the Protein Society · 2021
    Review
  11. Article
  12. The hydrodynamic motion of Nanodiscs.Chemistry and physics of lipids · 2019
    Article
  13. Review
  14. Article
  15. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Meg TraheyDepartment of Biomedical and Pharmaceutical Sciences, University of Montana, Missoula, Montana.
Mavis Jiarong LiDepartment of Medicinal Chemistry, University of Washington, Seattle Washington.
Hyewon KwonDepartment of Medicinal Chemistry, University of Washington, Seattle Washington.
Erica L WoodahlDepartment of Biomedical and Pharmaceutical Sciences, University of Montana, Missoula, Montana.
Wynton D McClaryDepartment of Medicinal Chemistry, University of Washington, Seattle Washington.
William M AtkinsDepartment of Medicinal Chemistry, University of Washington, Seattle Washington.
University of Washington · USUniversity of Montana · US

Funding

Surveillance genome sequencing to detect SARS-CoV-2 virus variants in MontanaP20GM103546 · NIGMS · UNIVERSITY OF MONTANA · PI BOWLER, BRUCE E · 2012 to 2021
$19.2M
Pharmacological Sciences SupplementT32GM007750 · NIGMS · UNIVERSITY OF WASHINGTON · PI ATKINS, WILLIAM M · 1985 to 2023
$12.6M
P450-Base Drug Interactions with Low Spin DrugsR01GM110790 · NIGMS · UNIVERSITY OF WASHINGTON · PI ATKINS, WILLIAM M · 2013 to 2016
$1.7M
Molecular Mechanisms of P-GlycoproteinR01GM098457 · NIGMS · UNIVERSITY OF WASHINGTON · PI ATKINS, WILLIAM M · 2011 to 2014
$1.1M
NIGMS NIH HHS 1R01 GM098457NIGMS NIH HHS 1R01 GM110790NIGMS NIH HHS P20 GM103546NIGMS NIH HHS R01 GM098457NIGMS NIH HHS R01 GM110790NIGMS NIH HHS T32 GM007750
6 · The paper itself

Abstract

Methods for the initial steps of surface plasmon resonance analysis of membrane proteins incorporated in lipid nanodiscs are described. Several types of Biacore sensor chips are available and require distinct strategies to immobilize proteonanodiscs on the chip surface. The procedures for immobilization on three of these chips (NTA, antibody coupled CM5, and L1) are described in this unit and results are demonstrated for a model system with cytochrome P4503A4 (CYP3A4) in nanodiscs binding to a polyclonal anti-CYP3A4 antibody. Advantages and disadvantages of each chip type are considered.

Indexed as

Antibodies, ImmobilizedBiosensing TechniquesLipidsMembrane ProteinsNanostructuresSurface Plasmon ResonanceAntibodies, ImmobilizedLipidsMembrane Proteinsantibody-receptor bindingbiosensorlipid nanodiscmembrane proteinsurface plasmon resonance binding analysis

Identifiers

PMID26237675
PMCPMC4533999
OpenAlexW1960703364

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.