ArticleNature structural & molecular biology2015
Structure and mechanism of the Rubisco-assembly chaperone Raf1.
Article in Nature structural & molecular biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed.
- Functional and structural insights into cyanobacterial COThe Plant journal : for cell and molecular biology · 2026Review
- Evolution of protein domains and protein domain combinations provides insights into the origin and diversification of land plants.Cell genomics · 2026Article
- High-Throughput Detection of Cyanobacterial Form I Rubisco Assembly.ACS synthetic biology · 2026Article
- Not just Rubisco: integrated strategies to overcome the biochemical limitations of CFrontiers in plant science · 2026Review
- tRNA as an assembly chaperone for a macromolecular transcription-processing complex.Nature structural & molecular biology · 2025Article
- Strategies to improve photosynthesis by modifying the RuBisCO system and its limitations.Molecular biology reports · 2025Review
- Directed evolution of a plant Rubisco chaperone with altered client recognition.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Replacement of large subunit N terminus enabled biogenesis of different plant Rubiscos in E. coli.Plant biotechnology journal · 2025Article
- Engineering COPlant communications · 2025Article
- A systematic exploration of bacterial form I rubisco maximal carboxylation rates.The EMBO journal · 2024Article
- Improving Crop Yield through Increasing Carbon Gain and Reducing Carbon Loss.Plants (Basel, Switzerland) · 2024Review
- Engineering Rubisco to enhance COSynthetic and systems biotechnology · 2024Review
- DnaK and DnaJ proteins from Hsp70/40 family are involved in Rubisco biosynthesis in Synechocystis sp. PCC6803 and sustain the enzyme assembly in a heterologous system.BMC plant biology · 2023Article
- Structural insights into cyanobacterial RuBisCO assembly coordinated by two chaperones Raf1 and RbcX.Cell discovery · 2022Article
- A temporal gradient of cytonuclear coordination of chaperonins and chaperones during RuBisCo biogenesis in allopolyploid plants.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Overexpression ofGenes · 2022Article
- Rubiscosome gene expression is balanced across the hexaploid wheat genome.Photosynthesis research · 2022Article
- Incorporation of Functional Rubisco Activases into Engineered Carboxysomes to Enhance Carbon Fixation.ACS synthetic biology · 2022Article
- Scaffolding protein CcmM directs multiprotein phase separation in β-carboxysome biogenesis.Nature structural & molecular biology · 2021Article
- The state of oligomerization of Rubisco controls the rate of synthesis of the Rubisco large subunit in Chlamydomonas reinhardtii.The Plant cell · 2021Article
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Biogenesis of the photosynthetic enzyme Rubisco, a complex of eight large (RbcL) and eight small (RbcS) subunits, requires assembly chaperones. Here we analyzed the role of Rubisco accumulation factor1 (Raf1), a dimer of ∼40-kDa subunits. We find that Raf1 from Synechococcus elongatus acts downstream of chaperonin-assisted RbcL folding by stabilizing RbcL antiparallel dimers for assembly into RbcL8 complexes with four Raf1 dimers bound. Raf1 displacement by RbcS results in holoenzyme formation. Crystal structures show that Raf1 from Arabidopsis thaliana consists of a β-sheet dimerization domain and a flexibly linked α-helical domain. Chemical cross-linking and EM reconstruction indicate that the β-domains bind along the equator of each RbcL2 unit, and the α-helical domains embrace the top and bottom edges of RbcL2. Raf1 fulfills a role similar to that of the assembly chaperone RbcX, thus suggesting that functionally redundant factors ensure efficient Rubisco biogenesis.
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