ArticlePloS one2015
Generation of Functional Cardiomyocytes from Efficiently Generated Human iPSCs and a Novel Method of Measuring Contractility.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Metabolic fatty acid substrates enhance the structural and mechanical performance of human-induced pluripotent stem cell-derived cardiomyocytes.Cellular and molecular life sciences : CMLS · 2026Article
- Induced mesenchymal stem cells generated from periodontal ligament fibroblast for regenerative therapy.Experimental biology and medicine (Maywood, N.J.) · 2025Article
- scaRNA20 promotes pseudouridylatory modification of small nuclear snRNA U12 and improves cardiomyogenesis.Experimental cell research · 2024Article
- MicroBundleCompute: Automated segmentation, tracking, and analysis of subdomain deformation in cardiac microbundles.PloS one · 2024Article
- Hutchinson-Gilford progeria patient-derived cardiomyocyte model of carrying LMNA gene variant c.1824 C > T.Cell and tissue research · 2023Article
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- Comparative analysis of human induced pluripotent stem cell-derived mesenchymal stem cells and umbilical cord mesenchymal stem cells.Journal of cellular and molecular medicine · 2021Article
- Noonan syndrome patient-specific induced cardiomyocyte model carrying SOS1 gene variant c.1654A>G.Experimental cell research · 2021Article
- Spatiotemporal Characterizations of Spontaneously Beating Cardiomyocytes with Adaptive Reference Digital Image Correlation.Scientific reports · 2019Article
- Assessment of temporal functional changes and miRNA profiling of human iPSC-derived cardiomyocytes.Scientific reports · 2019Article
- Pre-Conditioning Stem Cells in a Biomimetic Environment for Enhanced Cardiac Tissue Repair:Cellular and molecular bioengineering · 2018Article
- Manipulation-free cultures of human iPSC-derived cardiomyocytes offer a novel screening method for cardiotoxicity.Acta pharmacologica Sinica · 2018Article
- An inactivating mutation in the histone deacetylase SIRT6 causes human perinatal lethality.Genes & development · 2018Article
- Optical-flow based non-invasive analysis of cardiomyocyte contractility.Scientific reports · 2017Article
- Optical Method to Quantify Mechanical Contraction and Calcium Transients of Human Pluripotent Stem Cell-Derived Cardiomyocytes.Tissue engineering. Part C, Methods · 2017Article
- Non-viral reprogramming and induced pluripotent stem cells for cardiovascular therapy.Differentiation; research in biological diversityReview
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Authors and funding
7 authors.
Funding
Abstract
Human induced pluripotent stem cells (iPSCs) derived cardiomyocytes (iCMCs) would provide an unlimited cell source for regenerative medicine and drug discoveries. The objective of our study is to generate functional cardiomyocytes from human iPSCs and to develop a novel method of measuring contractility of CMCs. In a series of experiments, adult human skin fibroblasts (HSF) and human umbilical vein endothelial cells (HUVECs) were treated with a combination of pluripotent gene DNA and mRNA under specific conditions. The iPSC colonies were identified and differentiated into various cell lineages, including CMCs. The contractile activity of CMCs was measured by a novel method of frame-by-frame cross correlation (particle image velocimetry-PIV) analysis. Our treatment regimen transformed 4% of HSFs into iPSC colonies at passage 0, a significantly improved efficiency compared with use of either DNA or mRNA alone. The iPSCs were capable of differentiating both in vitro and in vivo into endodermal, ectodermal and mesodermal cells, including CMCs with >88% of cells being positive for troponin T (CTT) and Gata4 by flow cytometry. We report a highly efficient combination of DNA and mRNA to generate iPSCs and functional iCMCs from adult human cells. We also report a novel approach to measure contractility of iCMCs.
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