Evidence map›Paper›PMID 26236493›Full record

ArticleNew microbes and new infections2015

International Mycoplasma pneumoniae typing study: interpretation of M. pneumoniae multilocus variable-number tandem-repeat analysis.

V J Chalker, S Pereyre, R Dumke, J Winchell, P Khosla, H Sun, C Yan, C Vink, C Bébéar

Open access · goldAbstract read
In one paragraph

Article in New microbes and new infections, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.

0numbers the graph read from it
0cells of the map it votes in
32citing papers in PubMed
4.1field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

32 citing papers in PubMed, 46 citations in OpenAlex.

  1. Real-Time PCR and Quantitative Culture forBioMed research international · 2020
    Trial
  2. Article
  3. Host inflammatory markers and MLVA profiling for risk assessment of severe pediatricFrontiers in cellular and infection microbiology · 2026
    Article
  4. Genetic factors driving theThe Lancet regional health. Western Pacific · 2025
    Article
  5. Article
  6. P1 adhesin genotype characteristics ofFrontiers in cellular and infection microbiology · 2025
    Article
  7. Article
  8. Article
  9. Molecular Tools for TypingFrontiers in microbiology · 2022
    Review
  10. Article
  11. Molecular characterization of Mycoplasma pneumoniae infections in Moscow from 2015 to 2018.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2020
    Article
  12. Article
  13. Periodic Genotype Shifts in Clinically PrevalentFrontiers in cellular and infection microbiology · 2020
    Article
  14. Mycoplasma pneumoniae outbreak, Southeastern Finland, 2017-2018: molecular epidemiology and laboratory diagnostic lessons.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2019
    Article
  15. Molecular Typing ofJournal of clinical microbiology · 2019
    Article
  16. First Description of Macrolide-ResistantBioMed research international · 2019
    Article
  17. Antimicrobial susceptibility and genotyping ofAntimicrobial resistance and infection control · 2019
    Article
  18. Antimicrobial susceptibility and molecular characteristics ofAntimicrobial resistance and infection control · 2019
    Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 6 institutions in 6 countries.

V J ChalkerPublic Health England Colindale, London, UK.
S PereyreUSC EA 3671 Mycoplasmal and Chlamydial Infections in Humans, University of Bordeaux, France ; USC EA 3671 Mycoplasmal and Chlamydial Infections in Humans, Institut National de la Recherche Agronomique, Bordeaux, France.
R DumkeTU Dresden, Medical Faculty Carl Gustav Carus, Institute of Medical Microbiology and Hygiene, Dresden, Germany.
J WinchellRespiratory Diseases Branch, Centers for Disease Control and Prevention, Atlanta, GA, USA.
P KhoslaPublic Health England Colindale, London, UK.
H SunDepartment of Bacteriology, Capital Institute of Pediatrics, Beijing, China.
C YanDepartment of Bacteriology, Capital Institute of Pediatrics, Beijing, China.
C VinkErasmus MC-Sophia Children's Hospital, Laboratory of Pediatrics, Rotterdam, The Netherlands.
C BébéarUSC EA 3671 Mycoplasmal and Chlamydial Infections in Humans, University of Bordeaux, France ; USC EA 3671 Mycoplasmal and Chlamydial Infections in Humans, Institut National de la Recherche Agronomique, Bordeaux, France.
Capital Institute of Pediatrics · CNInfectiologie Animale et Santé Publique · FRPublic Health England · GBCenters for Disease Control and Prevention · USErasmus MC - Sophia Children’s Hospital · NLTU Dresden · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Typing of Mycoplasma pneumoniae by multiple-locus variable-number tandem repeat analysis (MLVA) is increasingly in use. However, no specific internationally agreed guidance is available. Thirty M. pneumoniae DNA samples including serial dilutions of a type strain were sent to six international laboratories to perform MLVA and results were compared. Good correlation was observed, indicating that this methodology can be robustly performed in multiple sites. However, differences due to interpretation of fragment size, repeat sequence identification and repeat numbering led to inconsistency in the final profiles assigned by laboratories. We propose guidelines for interpreting M. pneumoniae MLVA typing and assigning the number of repeats.

Indexed as

Interpretation guidelinesmolecular typingmultiple-locus variable-number tandem repeat analysis (MLVA)Mycoplasma pneumoniaestandardisation

Identifiers

PMID26236493
PMCPMC4501435
OpenAlexW2124333032

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.