ArticleBiotechnology for biofuels2015
Alleviation of carbon catabolite repression in Enterobacter aerogenes for efficient utilization of sugarcane molasses for 2,3-butanediol production.
Article in Biotechnology for biofuels, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed, 48 citations in OpenAlex.
- Adaptive Laboratory Evolution of the Non-Conventional YeastMicroorganisms · 2026Article
- Development of a high-throughput label-free method for enrichment of antimetabolite-resistant yeast strains using pico-litre agarose beads.Applied microbiology and biotechnology · 2026Article
- Predictive dynamic control accurately maps the design space for 2,3-butanediol production.Computational and structural biotechnology journal · 2024Article
- Insights into the regulatory mechanisms and application prospects of the transcription factor Cra.Applied and environmental microbiology · 2024Review
- Synthetic redesign of Escherichia coli W for faster metabolism of sugarcane molasses.Microbial cell factories · 2024Article
- Systemic metabolic engineering of Enterobacter aerogenes for efficient 2,3-butanediol production.Applied microbiology and biotechnology · 2024Article
- Diverse Virulence Attributes ofInternational journal of molecular sciences · 2023Article
- Highly efficient production of 2,3-butanediol from xylose and glucose by newly isolated thermotolerant Cronobacter sakazakii.BMC microbiology · 2022Article
- Effectively Converting Cane Molasses into 2,3-Butanediol UsingMolecules (Basel, Switzerland) · 2022Article
- Resveratrol production of a recombinantBiotechnology notes (Amsterdam, Netherlands) · 2022Article
- The current strategies and parameters for the enhanced microbial production of 2,3-butanediol.Biotechnology reports (Amsterdam, Netherlands) · 2020Article
- Generation of an E. coli platform strain for improved sucrose utilization using adaptive laboratory evolution.Microbial cell factories · 2019Article
- Engineered E. coli W enables efficient 2,3-butanediol production from glucose and sugar beet molasses using defined minimal medium as economic basis.Microbial cell factories · 2018Article
- Production of (2R, 3R)-2,3-butanediol using engineeredBiotechnology for biofuels · 2018Article
- Purification of 2,3-butanediol from fermentation broth: process development and techno-economic analysis.Biotechnology for biofuels · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundDue to its cost-effectiveness and rich sugar composition, sugarcane molasses is considered to be a promising carbon source for biorefinery. However, the sugar mixture in sugarcane molasses is not consumed as efficiently as glucose in microbial fermentation due to complex interactions among their utilizing pathways, such as carbon catabolite repression (CCR). In this study, 2,3-butanediol-producing Enterobacter aerogenes was engineered to alleviate CCR and improve sugar utilization by modulating its carbon preference.
resultsThe gene encoding catabolite repressor/activator (Cra) was deleted in the genome of E. aerogenes to increase the fructose consumption rate. However, the deletion mutation repressed sucrose utilization, resulting in the accumulation of sucrose in the fermentation medium. Cra regulation on expression of the scrAB operon involved in sucrose catabolism was verified by reverse transcription and real-time PCR, and the efficiency of sucrose utilization was restored by disrupting the scrR gene and overexpressing the scrAB operon. In addition, overexpression of the ptsG gene involved in glucose utilization enhanced the glucose preference among mixed sugars, which relieved glucose accumulation in fed-batch fermentation. In fed-batch fermentation using sugarcane molasses, the maximum titer of 2,3-butanediol production by the mutant reached 140.0 g/L at 54 h, which was by far the highest titer of 2,3-butanediol with E. aerogenes achieved through genetic engineering.
conclusionsWe have developed genetically engineered E. aerogenes as a 2,3-butanediol producer that efficiently utilizes sugarcane molasses. The fermentation efficiency was dramatically improved by the alleviation of CCR and modulation of carbon preference. These results offer a metabolic engineering approach for achieving highly efficient utilization of mixed sugars for the biorefinery industry.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.