ArticleFrontiers in microbiology2015
A flexible and economical barcoding approach for highly multiplexed amplicon sequencing of diverse target genes.
Article in Frontiers in microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 94 papers, 1 of them a synthesis that pooled it.
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Who cites it
94 citing papers in PubMed, 1 synthesis or guideline pooled it.
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- Lactulose selectively stimulates members of the gut microbiota, as determined by multi-modal activity-based sorting.Gut microbes · 2025Article
- Sulfoquinovose is exclusively metabolized by the gut microbiota and degraded differently in mice and humans.Microbiome · 2025Article
- The Parkinson's disease drug entacapone disrupts gut microbiome homoeostasis via iron sequestration.Nature microbiology · 2024Article
- Optimal liquid-based DNA preservation for DNA barcoding of field-collected fungal specimens.Heliyon · 2024Article
- Effects of Short-Term Tillage on Rhizosphere Soil Nitrogen Mineralization and Microbial Community Composition in Double-Cropping Rice Field.Journal of microbiology and biotechnology · 2024Article
- Plant roots affect free-living diazotroph communities in temperate grassland soils despite decades of fertilization.Communications biology · 2024Article
- The maternal microbiome in pregnancy, delivery, and early-stage development of neonatal microbiome after cesarean section: A prospective longitudinal study.Acta obstetricia et gynecologica Scandinavica · 2024Observational
- Comparison of Rhizosphere Bacterial Communities ofMicroorganisms · 2024Article
- A metalloprotease secreted by an environmentally acquired gut bacterium hindersFrontiers in cellular and infection microbiology · 2024Article
- Identification of inulin-responsive bacteria in the gut microbiota via multi-modal activity-based sorting.Nature communications · 2023Article
- Bacterial diversity in arboreal ant nesting spaces is linked to colony developmental stage.Communications biology · 2023Article
- The Parkinson's drug entacapone disrupts gut microbiome homeostasis via iron sequestration.bioRxiv : the preprint server for biology · 2023Article
- 24-Norursodeoxycholic acid ameliorates experimental alcohol-related liver disease and activates hepatic PPARγ.JHEP reports : innovation in hepatology · 2023Article
- Identification of Bacterial Taxa Present in a Concrete Pennsylvania Bridge.Microbiology resource announcements · 2023Article
- Rapid nitrification involving comammox and canonical Nitrospira at extreme pH in saline-alkaline lakes.Environmental microbiology · 2023Article
34 more citing papers are in PubMed but not listed here.
Corrections and comments
- Erratum issued
Authors and funding
7 authors.
Funding
Abstract
High throughput sequencing of phylogenetic and functional gene amplicons provides tremendous insight into the structure and functional potential of complex microbial communities. Here, we introduce a highly adaptable and economical PCR approach to barcoding and pooling libraries of numerous target genes. In this approach, we replace gene- and sequencing platform-specific fusion primers with general, interchangeable barcoding primers, enabling nearly limitless customized barcode-primer combinations. Compared to barcoding with long fusion primers, our multiple-target gene approach is more economical because it overall requires lower number of primers and is based on short primers with generally lower synthesis and purification costs. To highlight our approach, we pooled over 900 different small-subunit rRNA and functional gene amplicon libraries obtained from various environmental or host-associated microbial community samples into a single, paired-end Illumina MiSeq run. Although the amplicon regions ranged in size from approximately 290 to 720 bp, we found no significant systematic sequencing bias related to amplicon length or gene target. Our results indicate that this flexible multiplexing approach produces large, diverse, and high quality sets of amplicon sequence data for modern studies in microbial ecology.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.