ArticleFrontiers in microbiology2015
Marinobacter subterrani, a genetically tractable neutrophilic Fe(II)-oxidizing strain isolated from the Soudan Iron Mine.
Article in Frontiers in microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 51 citations in OpenAlex.
- Structural color in the bacterial domain: The ecogenomics of a 2-dimensional optical phenotype.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- The Genomic-Driven Discovery of Glutarimide-Containing Derivatives fromMolecules (Basel, Switzerland) · 2023Article
- Marinobacter: A case study in bioelectrochemical chassis evaluation.Microbial biotechnology · 2023Review
- Leader peptide removal in lasso peptide biosynthesis based on penultimate isoleucine residue.Frontiers in microbiology · 2023Article
- Diversity of Mixotrophic Neutrophilic Thiosulfate- and Iron-Oxidizing Bacteria from Deep-Sea Hydrothermal Vents.Microorganisms · 2022Article
- Metagenomic Features Characterized with Microbial Iron Oxidoreduction and Mineral Interaction in Southwest Indian Ridge.Microbiology spectrum · 2022Article
- Salinity Impact on Composition and Activity of Nitrate-Reducing Fe(II)-Oxidizing Microorganisms in Saline Lakes.Applied and environmental microbiology · 2022Article
- Evolutionary Divergence ofFrontiers in microbiology · 2022Article
- Bacteria Associated WithFrontiers in microbiology · 2022Article
- Article
- Oligo-heterotrophic Activity ofApplied and environmental microbiology · 2021Article
- Characterization ofMicroorganisms · 2021Article
- Rational construction of genome-reduced Burkholderiales chassis facilitates efficient heterologous production of natural products from proteobacteria.Nature communications · 2021Article
- Novel Microbial Groups Drive Productivity in an Archean Iron Formation.Frontiers in microbiology · 2021Article
- Metagenomic Insights Into the Diversity of Halophilic Microorganisms Indigenous to the Karak Salt Mine, Pakistan.Frontiers in microbiology · 2020Article
- Structure and Function of the Acetylpolyamine Amidohydrolase from the Deep Earth HalophileBiochemistry · 2019Article
- Electrical energy storage with engineered biological systems.Journal of biological engineering · 2019Review
- Differences in Applied Redox Potential on Cathodes Enrich for Diverse Electrochemically Active Microbial Isolates From a Marine Sediment.Frontiers in microbiology · 2019Article
- Whole Genome Sequencing and Metabolomic Study of CaveFrontiers in microbiology · 2019Article
- Draft Genome Sequences of Marinobacter Strains Recovered from Utica Shale-Produced Fluids.Genome announcements · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We report the isolation, characterization, and development of a robust genetic system for a halophilic, Fe(II)-oxidizing bacterium isolated from a vertical borehole originating 714 m below the surface located in the Soudan Iron Mine in northern Minnesota, USA. Sequence analysis of the 16S rRNA gene places the isolate in the genus Marinobacter of the Gammaproteobacteria. The genome of the isolate was sequenced using a combination of short- and long-read technologies resulting in two contigs representing a 4.4 Mbp genome. Using genomic information, we used a suicide vector for targeted deletion of specific flagellin genes, resulting in a motility-deficient mutant. The motility mutant was successfully complemented by expression of the deleted genes in trans. Random mutagenesis using a transposon was also achieved. Capable of heterotrophic growth, this isolate represents a microaerophilic Fe(II)-oxidizing species for which a system for both directed and random mutagenesis has been established. Analysis of 16S rDNA suggests Marinobacter represents a major taxon in the mine, and genetic interrogation of this genus may offer insight into the structure of deep subsurface communities as well as an additional tool for analyzing nutrient and element cycling in the subsurface ecosystem.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.