Evidence map›Paper›PMID 26235893›Full record

ArticleStem cell reports2015

α-5 Laminin Synthesized by Human Pluripotent Stem Cells Promotes Self-Renewal.

Alex Laperle, Cheston Hsiao, Michael Lampe, Jaime Mortier, Krishanu Saha, Sean P Palecek, Kristyn S Masters

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.

0numbers the graph read from it
0cells of the map it votes in
38citing papers in PubMed
3.6field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

38 citing papers in PubMed, 68 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Alex LaperleDepartment of Biomedical Engineering, University of Wisconsin - Madison, 1550 Engineering Drive, Madison, WI 53706, USA; Wisconsin Institute for Discovery, University of Wisconsin - Madison, 330 North Orchard Street, Madison, WI 53715, USA.
Cheston HsiaoDepartment of Chemical and Biological Engineering, University of Wisconsin - Madison, 1415 Engineering Drive, Madison, WI 53706, USA.
Michael LampeDepartment of Chemical and Biological Engineering, University of Wisconsin - Madison, 1415 Engineering Drive, Madison, WI 53706, USA.
Jaime MortierDepartment of Biomedical Engineering, University of Wisconsin - Madison, 1550 Engineering Drive, Madison, WI 53706, USA.
Krishanu SahaDepartment of Biomedical Engineering, University of Wisconsin - Madison, 1550 Engineering Drive, Madison, WI 53706, USA; Wisconsin Institute for Discovery, University of Wisconsin - Madison, 330 North Orchard Street, Madison, WI 53715, USA.
Sean P PalecekDepartment of Biomedical Engineering, University of Wisconsin - Madison, 1550 Engineering Drive, Madison, WI 53706, USA; Department of Chemical and Biological Engineering, University of Wisconsin - Madison, 1415 Engineering Drive, Madison, WI 53706, USA.
Kristyn S MastersDepartment of Biomedical Engineering, University of Wisconsin - Madison, 1550 Engineering Drive, Madison, WI 53706, USA; Wisconsin Institute for Discovery, University of Wisconsin - Madison, 330 North Orchard Street, Madison, WI 53715, USA. Electronic address: kmasters@wisc.edu.
University of Wisconsin–Madison · US

Funding

Regulating human pluripotent stem cell differentiation by colony confinementR01EB007534 · NIBIB · UNIVERSITY OF WISCONSIN-MADISON · PI PALECEK, SEAN P · 2007 to 2019
$4.1M
Creating Engineered Models of Valvular Disease to Study Anti-Calcific TherapiesR01HL093281 · NHLBI · UNIVERSITY OF WISCONSIN-MADISON · PI MASTERS, KRISTYN S · 2009 to 2014
$1.6M
NHLBI NIH HHS R01 HL093281NIBIB NIH HHS R01 EB007534
6 · The paper itself

Abstract

Substrate composition significantly impacts human pluripotent stem cell (hPSC) self-renewal and differentiation, but relatively little is known about the role of endogenously produced extracellular matrix (ECM) components in regulating hPSC fates. Here we identify α-5 laminin as a signature ECM component endogenously synthesized by undifferentiated hPSCs cultured on defined substrates. Inducible shRNA knockdown and Cas9-mediated disruption of the LAMA5 gene dramatically reduced hPSC self-renewal and increased apoptosis without affecting the expression of pluripotency markers. Increased self-renewal and survival was restored to wild-type levels by culturing the LAMA5-deficient cells on exogenous laminin-521. Furthermore, treatment of LAMA5-deficient cells with blebbistatin or a ROCK inhibitor partially restored self-renewal and diminished apoptosis. These results demonstrate that endogenous α-5 laminin promotes hPSC self-renewal in an autocrine and paracrine manner. This finding has implications for understanding how stem cells dynamically regulate their microenvironment to promote self-renewal and provides guidance for efforts to design substrates for stem cell bioprocessing.

Indexed as

Cell ProliferationApoptosisCell LineHumansLamininPluripotent Stem CellsLamininlaminin alpha5

Identifiers

PMID26235893
PMCPMC4618661
OpenAlexW1537475638

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.