ArticleScientific reports2015
Self-assembled Messenger RNA Nanoparticles (mRNA-NPs) for Efficient Gene Expression.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed.
- GENRISE-induced superior extracellular vesicles for scalable therapeutic cargo delivery.Science advances · 2025Article
- Peptides: potential delivery systems for mRNA.RSC chemical biology · 2025Review
- Single-molecule RNA sizing enables quantitative analysis of alternative transcription termination.Nature communications · 2024Article
- Making the Next Generation of Therapeutics: mRNA Meets Synthetic Biology.ACS synthetic biology · 2023Review
- Endothelial cell direct reprogramming: Past, present, and future.Journal of molecular and cellular cardiology · 2023Review
- Nucleic acid nanoassembly-enhanced RNA therapeutics and diagnosis.Acta pharmaceutica Sinica. B · 2023Review
- Towards novel nano-based vaccine platforms for SARS-CoV-2 and its variants of concern: Advances, challenges and limitations.Journal of drug delivery science and technology · 2022Review
- Nanoparticles in clinical trials of COVID-19: An update.International journal of surgery (London, England) · 2022Review
- Multimeric RNAs for efficient RNA-based therapeutics and vaccines.Journal of controlled release : official journal of the Controlled Release Society · 2022Review
- Development of a novel PTD-mediated IVT-mRNA delivery platform for potential protein replacement therapy of metabolic/genetic disorders.Molecular therapy. Nucleic acids · 2021Article
- RNA sequence and structure control assembly and function of RNA condensates.RNA (New York, N.Y.) · 2021Article
- Nano-carriers of COVID-19 vaccines: the main pillars of efficacy.Nanomedicine (London, England) · 2021Review
- Article
- RNA Nanotechnology to Solubilize Hydrophobic Antitumor Drug for Targeted Delivery.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2019Article
- Construction of RNA nanotubes.Nano research · 2019Article
- Numerical operations in living cells by programmable RNA devices.Science advances · 2019Article
- Article
- Delivering the Messenger: Advances in Technologies for Therapeutic mRNA Delivery.Molecular therapy : the journal of the American Society of Gene Therapy · 2019Review
- Nanoscale platforms for messenger RNA delivery.Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology · 2019Review
- Nanoparticle-Based Delivery of CRISPR/Cas9 Genome-Editing Therapeutics.The AAPS journal · 2018Review
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although mRNA has several advantages over plasmid DNA when delivered into cells for gene expression, mRNA transfection is a very rare occurrence in gene delivery. This is mainly because of the labile nature of RNA, resulting in a low expression level of the desired protein. In this study, self-assembled mRNA nanoparticles (mRNA-NPs) packed with multiple repeats of mRNA were synthesized to achieve efficient gene expression. This approach required only a one-step process to synthesize particles with a minimal amount of plasmid DNA to produce the RNA transcripts via rolling circle transcription. Moreover, there are no concerns for cytotoxicity which can be caused by chemical condensates because mRNA-NPs are made entirely of mRNA. An examination of the cells transfected with the mRNA-NPs encoding the green fluorescence protein (GFP) confirmed that the mRNA-NPs can be used as a novel platform for effective gene delivery.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.