ArticleNature communications2015
The mechanochemistry of copper reports on the directionality of unfolding in model cupredoxin proteins.
Article in Nature communications, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 66 citations in OpenAlex.
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- Advances in single-molecule junctions as tools for chemical and biochemical analysis.Nature chemistry · 2023Review
- Bond breaking of furan-maleimide adductsChemical science · 2023Article
- New insights into the folding-unfolding mechanism and conformations of cytochrome C.Chemical science · 2022Article
- An unexpected all-metal aromatic tetranuclear silver cluster in human copper chaperone Atox1.Chemical science · 2022Article
- Highly Dynamic Polynuclear Metal Cluster Revealed in a Single Metallothionein Molecule.Research (Washington, D.C.) · 2021Article
- Toward Cancer Diagnostics of the Tumor Suppressor p53 by Surface Enhanced Raman Spectroscopy.Sensors (Basel, Switzerland) · 2020Review
- Tuning Single-Molecule Conductance in Metalloporphyrin-Based Wires via Supramolecular Interactions.Angewandte Chemie (International ed. in English) · 2020Article
- Cation-induced shape programming and morphing in protein-based hydrogels.Science advances · 2020Article
- Electrochemical impedance biosensor array based on DNAzyme-functionalized single-walled carbon nanotubes using Gaussian process regression for Cu(II) and Hg(II) determination.Mikrochimica acta · 2020Article
- Depicting Conformational Ensembles of α-Synuclein by Single Molecule Force Spectroscopy and Native Mass Spectroscopy.International journal of molecular sciences · 2019Article
- Tuning Structure and Dynamics of Blue Copper Azurin Junctions via Single Amino-Acid Mutations.Biomolecules · 2019Article
- Mechanical Deformation and Electronic Structure of a Blue Copper Azurin in a Solid-State Junction.Biomolecules · 2019Article
- Multistep Protein Unfolding Scenarios from the Rupture of a Complex Metal Cluster CdScientific reports · 2019Article
- Enzymatic biosynthesis and immobilization of polyprotein verified at the single-molecule level.Nature communications · 2019Article
- Forcing the reversibility of a mechanochemical reaction.Nature communications · 2018Article
- Differences in the mechanical unfolding pathways of apo- and copper-bound azurins.Scientific reports · 2018Article
- Thy-1, a Pathfinder Protein for the Post-genomic Era.Frontiers in cell and developmental biology · 2018Review
- Copper (II) binding of NAD(P)H- flavin oxidoreductase (NfoR) enhances its Cr (VI)-reducing ability.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Understanding the directionality and sequence of protein unfolding is crucial to elucidate the underlying folding free energy landscape. An extra layer of complexity is added in metalloproteins, where a metal cofactor participates in the correct, functional fold of the protein. However, the precise mechanisms by which organometallic interactions are dynamically broken and reformed on (un)folding are largely unknown. Here we use single molecule force spectroscopy AFM combined with protein engineering and MD simulations to study the individual unfolding pathways of the blue-copper proteins azurin and plastocyanin. Using the nanomechanical properties of the native copper centre as a structurally embedded molecular reporter, we demonstrate that both proteins unfold via two independent, competing pathways. Our results provide experimental evidence of a novel kinetic partitioning scenario whereby the protein can stochastically unfold through two distinct main transition states placed at the N and C termini that dictate the direction in which unfolding occurs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.