ArticleJournal of industrial microbiology & biotechnology2015
Functional manipulations of the tetramycin positive regulatory gene ttmRIV to enhance the production of tetramycin A and nystatin A1 in Streptomyces ahygroscopicus.
Article in Journal of industrial microbiology & biotechnology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Research and Application of the Polyene Macrolide Antibiotic Nystatin.Molecules (Basel, Switzerland) · 2026Review
- Positive regulation of a LuxR family protein, MilO, in mildiomycin biosynthesis.Applied and environmental microbiology · 2025Article
- Comprehensive analysis of the mechanisms conferring resistance to phenamacril in theFrontiers in cellular and infection microbiology · 2025Review
- Transcriptional regulators of secondary metabolite biosynthesis in Streptomyces.World journal of microbiology & biotechnology · 2024Review
- Manipulation and epigenetic control of silent biosynthetic pathways in actinobacteria.World journal of microbiology & biotechnology · 2024Review
- Physiology and Transcriptional Analysis of ppGpp-Related Regulatory Effects in Streptomyces diastatochromogenes 1628.Microbiology spectrum · 2023Article
- Effect of PAS-LuxR Family Regulators on the Secondary Metabolism ofAntibiotics (Basel, Switzerland) · 2022Review
- Roles of LuxR-family regulators in the biosynthesis of secondary metabolites in Actinobacteria.World journal of microbiology & biotechnology · 2022Review
- Biosynthetic Potential ofAntibiotics (Basel, Switzerland) · 2021Article
- Optimization of tetramycin production in Streptomyces ahygroscopicus S91.Journal of biological engineering · 2021Article
- Production, Detection, Extraction, and Quantification of Polyene Antibiotics.Methods in molecular biology (Clifton, N.J.) · 2021Article
- The Application of Regulatory Cascades inFrontiers in microbiology · 2020Review
- Transcriptome-Based Identification of a Strong Promoter for Hyper-production of Natamycin in Streptomyces.Current microbiology · 2019Article
- Effects ofJournal of Zhejiang University. Science. BArticle
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7 authors.
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Abstract
A putative regulatory gene ttmRIV located in the tetramycin biosynthetic gene cluster was found in Streptomyces ahygroscopicus. In-frame deletion of ttmRIV led to abolishment of tetramycin and significant enhancement of nystatin A1, whose production reached 2.1-fold of the H42 parental strain. Gene complementation by an integrative plasmid carrying ttmRIV restored tetramycin biosynthesis revealed that ttmRIV was indispensable to tetramycin biosynthesis. Gene expression analysis of the H42 strain and its mutant strain ΔttmRIV via reverse transcriptase-PCR of the tetramycin gene cluster demonstrated that the expression levels of most biosynthetic genes were reduced in ΔttmRIV. Results of electrophoretic mobility shift assays showed that TtmRIV bound the putative promoters of several genes in the tetramycin pathway. Thus, TtmRIV is a pathway-specific positive regulator activating the transcription of the tetramycin gene cluster in S. ahygroscopicus. Providing an additional copy of ttmRIV under the control of the ermEp* promoter in the H42 strain boosted tetramycin A production to 3.3-fold.
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