Evidence map›Paper›PMID 26232787›Full record

ArticleThe international journal of neuropsychopharmacology2015

Prefrontal microRNA-221 Mediates Environmental Enrichment-Induced Increase of Locomotor Sensitivity to Nicotine.

Adrian M Gomez, Diego Altomare, Wei-Lun Sun, Narasimha M Midde, Hao Ji, Michael Shtutman, Jill R Turner, Kim E Creek, Jun Zhu

Erratum issuedOpen access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The international journal of neuropsychopharmacology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 11 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed, 2 pooled it
0.4field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 2 syntheses or guidelines pooled it, 13 citations in OpenAlex.

  1. Pooled it
  2. Role of microRNAs in the pathophysiology of addiction.Wiley interdisciplinary reviews. RNA · 2021
    Pooled it
  3. Review
  4. Article
  5. Article
  6. Review
  7. Review
  8. Noncoding RNA therapeutics for substance use disorder.Advances in drug and alcohol research · 2022
    Article
  9. Article
  10. Article
  11. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Adrian M GomezDepartment of Drug Discovery and Biomedical Sciences, South Carolina College of Pharmacy, University of South Carolina, Columbia, SC (Dr Gomez, Dr Altomare, Dr Sun, Dr Middle, Mrs Ji, Dr Shtutman, Dr Turner, Dr Creek, and Dr Zhu).
Diego AltomareDepartment of Drug Discovery and Biomedical Sciences, South Carolina College of Pharmacy, University of South Carolina, Columbia, SC (Dr Gomez, Dr Altomare, Dr Sun, Dr Middle, Mrs Ji, Dr Shtutman, Dr Turner, Dr Creek, and Dr Zhu).
Wei-Lun SunDepartment of Drug Discovery and Biomedical Sciences, South Carolina College of Pharmacy, University of South Carolina, Columbia, SC (Dr Gomez, Dr Altomare, Dr Sun, Dr Middle, Mrs Ji, Dr Shtutman, Dr Turner, Dr Creek, and Dr Zhu).
Narasimha M MiddeDepartment of Drug Discovery and Biomedical Sciences, South Carolina College of Pharmacy, University of South Carolina, Columbia, SC (Dr Gomez, Dr Altomare, Dr Sun, Dr Middle, Mrs Ji, Dr Shtutman, Dr Turner, Dr Creek, and Dr Zhu).
Hao JiDepartment of Drug Discovery and Biomedical Sciences, South Carolina College of Pharmacy, University of South Carolina, Columbia, SC (Dr Gomez, Dr Altomare, Dr Sun, Dr Middle, Mrs Ji, Dr Shtutman, Dr Turner, Dr Creek, and Dr Zhu).
Michael ShtutmanDepartment of Drug Discovery and Biomedical Sciences, South Carolina College of Pharmacy, University of South Carolina, Columbia, SC (Dr Gomez, Dr Altomare, Dr Sun, Dr Middle, Mrs Ji, Dr Shtutman, Dr Turner, Dr Creek, and Dr Zhu).
Jill R TurnerDepartment of Drug Discovery and Biomedical Sciences, South Carolina College of Pharmacy, University of South Carolina, Columbia, SC (Dr Gomez, Dr Altomare, Dr Sun, Dr Middle, Mrs Ji, Dr Shtutman, Dr Turner, Dr Creek, and Dr Zhu).
Kim E CreekDepartment of Drug Discovery and Biomedical Sciences, South Carolina College of Pharmacy, University of South Carolina, Columbia, SC (Dr Gomez, Dr Altomare, Dr Sun, Dr Middle, Mrs Ji, Dr Shtutman, Dr Turner, Dr Creek, and Dr Zhu).
Jun ZhuDepartment of Drug Discovery and Biomedical Sciences, South Carolina College of Pharmacy, University of South Carolina, Columbia, SC (Dr Gomez, Dr Altomare, Dr Sun, Dr Middle, Mrs Ji, Dr Shtutman, Dr Turner, Dr Creek, and Dr Zhu). zhuj@sccp.sc.edu.
Drew University · US

Funding

South Carolina IDeA Networks of Biomedical Research (SC INBRE V)P20GM103499 · NIGMS · UNIVERSITY OF SOUTH CAROLINA AT COLUMBIA · PI EDIE C GOLDSMITH · 2012 to 2026
$61.0M
Targeting NMDA Receptors and Brain Estradiol to Rescue Memory in Aging FemalesP20GM109091 · NIGMS · UNIVERSITY OF SOUTH CAROLINA AT COLUMBIA · PI KIARIS, HIPPOKRATIS, RONINSON, IGOR B · 2014 to 2024
$25.2M
Molecular mechanisms: Dysregulation of monoamine transporters by HIV-1Tat and cocaineR01DA035714 · NIDA · UNIVERSITY OF SOUTH CAROLINA AT COLUMBIA · PI Jay P. McLaughlin, CHANG-GUO ZHAN · 2013 to 2026
$6.6M
Pharmacogenomic Analysis of Nicotine DependenceR00DA032681 · NIDA · UNIVERSITY OF SOUTH CAROLINA AT COLUMBIA · PI TURNER, JILL R. · 2014 to 2016
$741k
Role of Dopamine Transporter: HIV-1 Tat Protein and Nicotine SensitizationR03DA026721 · NIDA · UNIVERSITY OF SOUTH CAROLINA AT COLUMBIA · PI ZHU, JUN · 2009 to 2010
$287k
Role of DARPP-32: Individual Responsiveness to NicotineR03DA024275 · NIDA · UNIVERSITY OF SOUTH CAROLINA AT COLUMBIA · PI ZHU, JUN · 2009 to 2010
$144k
NIDA NIH HHS R00 DA032681NIDA NIH HHS R01 DA035714NIDA NIH HHS R01DA035714NIDA NIH HHS R03 DA024275NIDA NIH HHS R03DA024275NIDA NIH HHS R03 DA026721NIDA NIH HHS R03DA026721NIGMS NIH HHS P20 GM103499NIGMS NIH HHS P20GM103499NIGMS NIH HHS P20 GM109091NIGMS NIH HHS P20GM109091
6 · The paper itself

Abstract

backgroundEnvironmental enrichment alters susceptibility in developing drug addiction. We have demonstrated that rats raised in an enriched condition are more sensitive than rats raised in an impoverished condition to nicotine-induced locomotor activity, and this is associated with alterations of phosphorylated extracellular signal-regulated kinase 1/2 within the prefrontal cortex. This study determined the impact of microRNA-221 in the prefrontal cortex on phosphorylated extracellular signal-regulated kinase 1/2 and the enriched environment-dependent behavioral changes in response to nicotine.

methodsA microRNA array was conducted to profile microRNA expression in the prefrontal cortex of enriched condition and impoverished condition rats in response to repeated nicotine (0.35 mg/kg, s.c.) administration. microRNA-221 in the prefrontal cortex, nucleus accumbens, and striatum was further verified by quantitative real-time PCR. Lentiviral-mediated overexpression of microRNA-221 in PC12 cells and the medial prefrontal cortex was performed to determine the effects of microRNA-221 on nicotine-mediated phosphorylated extracellular signal-regulated kinase 1/2, phosphorylated cAMP-response element-binding protein, and locomotor activity.

resultsmicroRNA-221 was profoundly upregulated in the prefrontal cortex but not in nucleus accumbens and striatum of enriched condition rats relative to impoverished condition rats following repeated administration of nicotine. Overexpression of lentiviral-microRNA-221 attenuated nicotine-induced increase in phosphorylated extracellular signal-regulated kinase 1/2 in PC12 cells. Lentiviral-microRNA-221 overexpression in the medial prefrontal cortex further increased locomotor activity in impoverished condition but not in enriched condition rats in response to repeated nicotine administration. Accordingly, lentiviral-microRNA-221 attenuated nicotine-induced increases in phosphorylated extracellular signal-regulated kinase 1/2 and phosphorylated cAMP-response element-binding protein in the medial prefrontal cortex of impoverished condition but not enriched condition rats.

conclusionThese findings suggest that environmental enrichment, via upregulation of prefrontal microRNA-221 expression, suppresses the nicotine-induced activation of extracellular signal-regulated kinase and cAMP-response element-binding protein, which provides a potential mechanism underlying enhanced locomotor sensitivity to nicotine.

Indexed as

EnvironmentAnimalsAnimals, NewbornComputational BiologyCREB-Binding ProteinGene Expression ProfilingHEK293 CellsHumansLocomotionMaleMAP Kinase Signaling SystemMicroRNAsNicotineNicotinic AgonistsOligonucleotide Array Sequence AnalysisPC12 CellsCREB-Binding ProteinMicroRNAsMIRN221 microRNA, ratMIRN483 microRNA, humanNicotineNicotinic AgonistsRNA, MessengerEnvironmental enrichmentERKmedial prefrontal cortexmicroRNAnicotine

Identifiers

PMID26232787
PMCPMC4772274
OpenAlexW2147059192

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.