ArticleGenetics2015
Efficient Genome Editing in Caenorhabditis elegans with a Toolkit of Dual-Marker Selection Cassettes.
Article in Genetics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.
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Who cites it
65 citing papers in PubMed.
- Domain-specific mutations in unc-6/Netrin differentially affect dorsal-ventral axon pathfinding in Caenorhabditis elegans.Biology open · 2026Article
- FLInt 2.0: robust and customizable single-shot integration in C. elegans.G3 (Bethesda, Md.) · 2026Article
- Domain-specific mutations inbioRxiv : the preprint server for biology · 2026Article
- Unbalanced chromatin binding of Polycomb complexes drives neurodevelopmental disorders.Molecular cell · 2026Article
- Programmed mitophagy at the oocyte-to-zygote transition promotes lineage endurance.Nature cell biology · 2026Article
- FLInt 2.0: Robust and customizable single shot integration inbioRxiv : the preprint server for biology · 2026Article
- Screening conditions and constructs for attempted genetic transformation of C. elegans by Agrobacterium.PloS one · 2025Article
- Robotic microinjection enables large-scale transgenic studies of Caenorhabditis elegans.Nature communications · 2024Article
- TDP-1 and FUST-1 co-inhibit exon inclusion and control fertility together with transcriptional regulation.Nucleic acids research · 2023Article
- A novel endoplasmic reticulum adaptation is critical for the long-lived Caenorhabditis elegans rpn-10 proteasomal mutant.Biochimica et biophysica acta. Gene regulatory mechanisms · 2023Article
- High-throughput phenotyping of infection by diverse microsporidia species reveals a wild C. elegans strain with opposing resistance and susceptibility traits.PLoS pathogens · 2023Article
- A Decade of CRISPR-Cas Gnome Editing inInternational journal of molecular sciences · 2022Review
- Channel-independent function of UNC-9/Innexin in spatial arrangement of GABAergic synapses ineLife · 2022Article
- Sensory neuron transcriptomes reveal complex neuron-specific function and regulation of mec-2/Stomatin splicing.Nucleic acids research · 2022Article
- Release of CHK-2 from PPM-1.D anchorage schedules meiotic entry.Science advances · 2022Article
- Article
- A role for the Erk MAPK pathway in modulating SAX-7/L1CAM-dependent locomotion in Caenorhabditis elegans.Genetics · 2022Article
- Approaches for CRISPR/Cas9 Genome Editing in C. elegans.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Identification of essential genes in Caenorhabditis elegans through whole-genome sequencing of legacy mutant collections.G3 (Bethesda, Md.) · 2021Article
- High-efficiency CRISPR gene editing in C. elegans using Cas9 integrated into the genome.PLoS genetics · 2021Article
5 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Use of the CRISPR/Cas9 RNA-guided endonuclease complex has recently enabled the generation of double-strand breaks virtually anywhere in the C. elegans genome. Here, we present an improved strategy that makes all steps in the genome editing process more efficient. We have created a toolkit of template-mediated repair cassettes that contain an antibiotic resistance gene to select for worms carrying the repair template and a fluorescent visual marker that facilitates identification of bona fide recombinant animals. Homozygous animals can be identified as early as 4-5 days post-injection, and minimal genotyping by PCR is required. We demonstrate that our toolkit of dual-marker vectors can generate targeted disruptions, deletions, and endogenous tagging with fluorescent proteins and epitopes. This strategy should be useful for a wide variety of additional applications and will provide researchers with increased flexibility when designing genome editing experiments.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.