ArticleIranian biomedical journal2015
Effects of Peptone Supplementation in Different Culture Media on Growth, Metabolic Pathway and Productivity of CHO DG44 Cells; a New Insight into Amino Acid Profiles.
Article in Iranian biomedical journal, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 35 citations in OpenAlex.
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- Characterization of the Antibacterial Activity of Quinone-Based Compounds Originating from the Alnumycin Biosynthetic Gene Cluster of aAntibiotics (Basel, Switzerland) · 2023Article
- Media optimization of antimicrobial activity production and beta-glucan content of endophytic fungiBiotechnology reports (Amsterdam, Netherlands) · 2022Article
- Applications and analysis of hydrolysates in animal cell culture.Bioresources and bioprocessing · 2021Review
- Facile development of medium optimization for antibody production: implementation in spinner flask and hollow fiber reactor.Cytotechnology · 2018Article
- Utilization of Site-Specific Recombination in Biopharmaceutical Production.Iranian biomedical journal · 2016Review
- Development of Low-Serum Culture Media for the in Vitro Cultivation ofIranian journal of parasitologyArticle
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe optimization of bioprocess conditions towards improved growth profile and productivity yield is considered of great importance in biopharmaceutical manufacturing. Peptones as efficient sources of nutrients have been studied for their effect on media development; however, their role on metabolic pathway is not well understood.
methodsIn the present study, the effect of different concentration of peptones on a recombinant Chinese hamster ovary (CHO) cell line grown in three serum-free suspension cultures was determined. Six peptones of different origins and available amino acid profiles were investigated regarding their impact on cell growth, productivity, and metabolic pathways changes.
resultsIn optimized feeding strategies, increases of 136% and 159% in volumetric productivity (for a low-nutrient culture media) and 55% (for a high-nutrient culture media) were achieved. Furthermore, particular sources of peptones with specific amino acid profile developed preferential results for each different culture medium. Two peptones, SoyA2SC and SoyE-110, were the only hydrolysates that showed production improvement in all three media. Casein Peptone plus Tryptone N1 and SoyA3SC showed different improved results based on their implemented concentration for each individual basal medium.
conclusionThe amino acid profile of peptones may provide clues to identify the most effective feeding strategies for recombinant CHO cells.
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