ArticleParasitology research2015
The siRNA-mediated silencing of Trichinella spiralis nudix hydrolase results in reduction of larval infectivity.
Article in Parasitology research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 56 citations in OpenAlex.
- Oral delivery of Trichinella spiralis antigens by yeast-derived β-glucan particles enhances anti-helminth immunity.NPJ vaccines · 2026Article
- In vitro knockdown of TsDNase II-7 suppresses Trichinella spiralis invasion into the host's intestinal epithelial cells.PLoS neglected tropical diseases · 2023Article
- Oral vaccination with recombinant Lactobacillus plantarum encoding Trichinella spiralis inorganic pyrophosphatase elicited a protective immunity in BALB/c mice.PLoS neglected tropical diseases · 2021Article
- RNAi-mediated silencing of Trichinella spiralis glutaminase results in reduced muscle larval infectivity.Veterinary research · 2021Article
- Biological properties and roles of a Trichinella spiralis inorganic pyrophosphatase in molting and developmental process of intestinal larval stages.Veterinary research · 2021Article
- Characterization of a Novel Glutamine Synthetase FromFrontiers in cell and developmental biology · 2021Article
- RNAi-mediated silencing of Trichinella spiralis serpin-type serine protease inhibitors results in a reduction in larval infectivity.Veterinary research · 2020Article
- Molecular characterization of a Trichinella spiralis serine proteinase.Veterinary research · 2020Article
- Vaccination of Mice with a Novel Trypsin fromVaccines · 2020Article
- Characterization of a Trichinella spiralis aminopeptidase and its participation in invasion, development and fecundity.Veterinary research · 2020Article
- Molecular characterization of a Trichinella spiralis aspartic protease and its facilitation role in larval invasion of host intestinal epithelial cells.PLoS neglected tropical diseases · 2020Article
- Haemonchus contortus: siRNA mediated knockdown of matrix metalloproteinase 12A (MMP-12) results in reduction of infectivity.Parasites & vectors · 2020Article
- Protective immunity in mice vaccinated with a novel elastase-1 significantly decreases Trichinella spiralis fecundity and infection.Veterinary research · 2020Article
- In vitro silencing of a serine protease inhibitor suppresses Trichinella spiralis invasion, development, and fecundity.Parasitology research · 2019Article
- Oral vaccination with Trichinella spiralis DNase II DNA vaccine delivered by attenuated Salmonella induces a protective immunity in BALB/c mice.Veterinary research · 2018Article
- Characterization of a Trichinella spiralis putative serine protease. Study of its potential as sero-diagnostic tool.PLoS neglected tropical diseases · 2018Article
- Screening and characterization of early diagnostic antigens in excretory-secretory proteins from Trichinella spiralis intestinal infective larvae by immunoproteomics.Parasitology research · 2016Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Previous studies showed that Trichinella spiralis Nudix hydrolase (TsNd) bound to intestinal epithelial cells (IECs), and vaccination of mice with rTsNd or TsNd DNA produced a partial protective immunity against T. spiralis infection. In this study, three TsNd specific small interfering RNA (siRNA) were designed to silence the expression of TsNd in T. spiralis larvae. SiRNAs were delivered to the larvae by electroporation. Silencing effect of TsNd transcription and expression was determined by real-time PCR and Western blotting, respectively. The infectivity of the larvae treated with siRNA was investigated by the in vitro larval invasion of IECs and experimental infection in mice. The results showed that siRNAs were efficiently delivered into T. spiralis larvae through electroporation. Real-time PCR and Western blotting showed that transcription and expression level of TsNd gene was inhibited 73.3 and 76.7 %, respectively, after being electroporated with 2 μM of siRNA-275 for 1 day. Silencing TsNd expression inhibited significantly the larval invasion of IECs (P < 0.01) and was in a dose-dependent manner (r = -0.97941). The mice with infected larvae treated with TsNd siRNA displayed a 63.6 % reduction in intestinal adult worms and 68.8 % reduction in muscle larval burden compared with mice infected with control siRNA-treated larvae. Our results showed that silencing TsNd expression in T. spiralis significantly reduced the larval infectivity and survival in host.
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