ArticleApplied and environmental microbiology2015
Burkholderia Diffusible Signal Factor Signals to Francisella novicida To Disperse Biofilm and Increase Siderophore Production.
Article in Applied and environmental microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 49 citations in OpenAlex.
- Characterization of carABpyrB operon and role of pyrE in Francisella novicida biofilm.MicrobiologyOpen · 2026Article
- Bacterial Biofilms-A Threat to Biliary Stents, Understanding Their Formation, Clinical Consequences and Management.Medicina (Kaunas, Lithuania) · 2025Review
- Fatty acid synthesis promoted by PA1895-1897 operon delays quorum sensing activation in Pseudomonas aeruginosa.AMB Express · 2024Article
- A 4-Hydroxybenzoic Acid-Mediated Signaling System Controls the Physiology and Virulence of Shigella sonnei.Microbiology spectrum · 2023Article
- Article
- Functionalized Self-Assembled Monolayers: Versatile Strategies to Combat Bacterial Biofilm Formation.Pharmaceutics · 2022Review
- Systematic identification of molecular mediators of interspecies sensing in a community of two frequently coinfecting bacterial pathogens.PLoS biology · 2022Article
- Diffusible signal factor signaling controls bioleaching activity and niche protection in the acidophilic, mineral-oxidizing leptospirilli.Scientific reports · 2021Article
- Metabolome Analysis of Constituents in Membrane Vesicles forMicroorganisms · 2021Article
- Genetic Determinants of Antibiotic Resistance inFrontiers in microbiology · 2021Review
- Natural products as inspiration for the development of bacterial antibiofilm agents.Natural product reports · 2020Review
- Biofilm dispersion.Nature reviews. Microbiology · 2020Review
- Targeting Biofilms Therapy: Current Research Strategies and Development Hurdles.Microorganisms · 2020Review
- Quorum sensing for population-level control of bacteria and potential therapeutic applications.Cellular and molecular life sciences : CMLS · 2020Review
- Biofilm architecture: An emerging synthetic biology target.Synthetic and systems biotechnology · 2020Review
- Francisella novicida and F. philomiragia biofilm features conditionning fitness in spring water and in presence of antibiotics.PloS one · 2020Article
- Quorum Quenching: A Potential Target for Antipseudomonal Therapy.Infection and drug resistance · 2020Review
- Structural basis of DSF recognition by its receptor RpfR and its regulatory interaction with the DSF synthase RpfF.PLoS biology · 2019Article
- Article
- Bifunctional Enzyme SpoT Is Involved in Biofilm Formation of Helicobacter pylori with Multidrug Resistance by Upregulating Efflux Pump Hp1174 (Antimicrobial agents and chemotherapy · 2018Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In many bacteria, the ability to modulate biofilm production relies on specific signaling molecules that are either self-produced or made by neighboring microbes within the ecological niche. We analyzed the potential interspecies signaling effect of the Burkholderia diffusible signal factor (BDSF) on Francisella novicida, a model organism for Francisella tularensis, and demonstrated that BDSF both inhibits the formation and causes the dispersion of Francisella biofilm. Specificity was demonstrated for the cis versus the trans form of BDSF. Using transcriptome sequencing, quantitative reverse transcription-PCR, and activity assays, we found that BDSF altered the expression of many F. novicida genes, including genes involved in biofilm formation, such as chitinases. Using a chitinase inhibitor, the antibiofilm activity of BDSF was also shown to be chitinase dependent. In addition, BDSF caused an increase in RelA expression and increased levels of (p)ppGpp, leading to decreased biofilm production. These results support our observation that exposure of F. novicida to BDSF causes biofilm dispersal. Furthermore, BDSF upregulated the genes involved in iron acquisition (figABCD), increasing siderophore production. Thus, this study provides evidence for a potential role and mechanism of diffusible signal factor (DSF) signaling in the genus Francisella and suggests the possibility of interspecies signaling between Francisella and other bacteria. Overall, this study suggests that in response to the interspecies DSF signal, F. novicida can alter its gene expression and regulate its biofilm formation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.