ArticleThe Journal of biological chemistry2015
Codon-Anticodon Recognition in the Bacillus subtilis glyQS T Box Riboswitch: RNA-DEPENDENT CODON SELECTION OUTSIDE THE RIBOSOME.
Article in The Journal of biological chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Structural idiosyncrasies of glycyl T-box riboswitches among pathogenic bacteria.RNA (New York, N.Y.) · 2024Article
- New Insights into Radio-Resistance Mechanism Revealed by (Phospho)Proteome Analysis ofInternational journal of molecular sciences · 2023Article
- Streptococcus suis TrpX is part of a tryptophan uptake system, and its expression is regulated by a T-box regulatory element.Scientific reports · 2022Article
- Cooperativity and Interdependency between RNA Structure and RNA-RNA Interactions.Non-coding RNA · 2021Review
- TBDB: a database of structurally annotated T-box riboswitch:tRNA pairs.Nucleic acids research · 2021Article
- Unboxing the T-box riboswitches-A glimpse into multivalent and multimodal RNA-RNA interactions.Wiley interdisciplinary reviews. RNA · 2020Review
- Structure-seq2 probing of RNA structure upon amino acid starvation reveals both known and novel RNA switches inRNA (New York, N.Y.) · 2020Article
- An aminoacylation ribozyme evolved from a natural tRNA-sensing T-box riboswitch.Nature chemical biology · 2020Article
- High-affinity recognition of specific tRNAs by an mRNA anticodon-binding groove.Nature structural & molecular biology · 2019Article
- An evolving tale of two interacting RNAs-themes and variations of the T-box riboswitch mechanism.IUBMB life · 2019Review
- The T-Box Riboswitch: tRNA as an Effector to Modulate Gene Regulation.Microbiology spectrum · 2018Review
- Molecular envelope and atomic model of an anti-terminated glyQS T-box regulator in complex with tRNAGly.Nucleic acids research · 2017Article
- Capture and Release of tRNA by the T-Loop Receptor in the Function of the T-Box Riboswitch.Biochemistry · 2017Article
- Article
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4 authors.
Funding
Abstract
Many amino acid-related genes in Gram-positive bacteria are regulated by the T box riboswitch. The leader RNA of genes in the T box family controls the expression of downstream genes by monitoring the aminoacylation status of the cognate tRNA. Previous studies identified a three-nucleotide codon, termed the "Specifier Sequence," in the riboswitch that corresponds to the amino acid identity of the downstream genes. Pairing of the Specifier Sequence with the anticodon of the cognate tRNA is the primary determinant of specific tRNA recognition. This interaction mimics codon-anticodon pairing in translation but occurs in the absence of the ribosome. The goal of the current study was to determine the effect of a full range of mismatches for comparison with codon recognition in translation. Mutations were individually introduced into the Specifier Sequence of the glyQS leader RNA and tRNA(Gly) anticodon to test the effect of all possible pairing combinations on tRNA binding affinity and antitermination efficiency. The functional role of the conserved purine 3' of the Specifier Sequence was also verifiedin this study. We found that substitutions at the Specifier Sequence resulted in reduced binding, the magnitude of which correlates well with the predicted stability of the RNA-RNA pairing. However, the tolerance for specific mismatches in antitermination was generally different from that during decoding, which reveals a unique tRNA recognition pattern in the T box antitermination system.
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